Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

Western Blot: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - Analysis of Cytokeratin 1 in Caco-2. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - Cytokeratin 1 antibody (LHK1) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) usi

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free [NBP2-80688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) usi

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: LHK1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free (NB100-2756) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: C-terminal peptide sequence of human Cytokeratin 1 [UniProt# P04264]
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2-10 ug/ml
  • Immunohistochemistry-Frozen 5-10 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: This Cytokeratin 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Immunohistochemistry-paraffin sections and Western blot.
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Cytokeratin 1 Antibody (LHK1) - Azide and BSA Free

• CK1
• CK-1
• cytokeratin 1
• Cytokeratin-1
• cytokeratin-1,67 kDa cytokeratin
• EHK
• EHK1
• epidermolytic hyperkeratosis 1
• EPPK
• Hair alpha protein
• K1
• keratin 1
• keratin, type II cytoskeletal 1
• Keratin-1
• KRT1A
• KRTA
• NEPPK
• Type-II keratin Kb1

Background

Cytokeratin 1 is a heterotetramer of two type I as well as two type II keratins localized in the cell membrane and is generally associated with keratin-10 (KRT10). It has been suggested to regulate the activity of kinases such as PKC/SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). Cytokeratin 1 and its heterodimer partner KRT10 are the major constituents of 4 intermediate filament cytoskeleton in suprabasal epidermis/keratinocytes, and their upregulation precedes expression of filaggrin and cornified envelope proteins. Covalent crosslinking of cytokeratin 1 but not of KRT10 to these proteins, in addition to a complex series of lipids, ultimately forms the cornified/lipid envelope, that constitutes the epidermal barrier together with Langerhans cells. Defects in cytokeratin 1 have been linked to epidermolytic hyperkeratosis (EHK), ichthyosis hystrix Curth-Macklin type (IHCM), palmoplantar keratoderma non-epidermolytic (NEPPK), ichthyosis annular epidermolytic (AEI), palmoplantar keratoderma striate type 3 (SPPK3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT1