Cytokeratin 1 Antibody (LHK1)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a ...read more
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a ...read more
Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Cytokeratin 1 antibody (LHK1) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
LHK1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.14 mg/ml

Cytokeratin 1 Antibody (LHK1) Summary

Immunogen
C-terminal peptide sequence of human Cytokeratin 1 [UniProt# P04264]
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2-10 ug/ml
  • Immunohistochemistry-Frozen 5-10 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
Application Notes
This Cytokeratin 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Immunohistochemistry-paraffin sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-2756 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.14 mg/ml
Purity
Protein G purified

Alternate Names for Cytokeratin 1 Antibody (LHK1)

  • CK1
  • CK-1
  • cytokeratin 1
  • Cytokeratin-1
  • cytokeratin-1,67 kDa cytokeratin
  • EHK
  • EHK1
  • epidermolytic hyperkeratosis 1
  • EPPK
  • Hair alpha protein
  • K1
  • keratin 1
  • keratin, type II cytoskeletal 1
  • Keratin-1
  • KRT1A
  • KRTA
  • NEPPK
  • Type-II keratin Kb1

Background

Cytokeratin 1 is a heterotetramer of two type I as well as two type II keratins localized in cell membrane generally associated with keratin-10 (KRT10), and it has been suggested to regulate the activity of kinases such as PKC/SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). Cytokeratin 1 and its heterodimer partner KRT10 are the major constituents of 4 intermediate filament cytoskeleton in suprabasal epidermis/keratinocytes, and their upregulation precedes expression of filaggrin and cornified envelope proteins. Covalent crosslinking of cytokeratin 1 but not of KRT10 to these proteins, in addition to a complex series of lipids, ultimately forms the cornified/lipid envelope, that constitutes the epidermal barrier together with Langerhans cells. Defects in cytokeratin 1 have been linked to epidermolytic hyperkeratosis (EHK), ichthyosis hystrix Curth-Macklin type (IHCM), palmoplantar keratoderma non-epidermolytic (NEPPK), ichthyosis annular epidermolytic (AEI), palmoplantar keratoderma striate type 3 (SPPK3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 1 Antibody (NB100-2756)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(5)
IHC
(3)
IHC-P
(2)
All Applications
Filter By Species
Human
(5)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-2756 Applications Species
Ha D, Bing SJ, Ahn G et al. Blocking Glutamate Carboxypeptidase II Inhibits Glutamate Excitotoxicity and Regulates Immune Responses in Experimental Autoimmune Encephalomyelitis FEBS J. 2016 Jul 22 [PMID: 27444540] (IHC, Human) IHC Human
McGovern JA, Meinert C, de Veer SJ et al. Attenuated kallikrein-related peptidase activity disrupts desquamation and leads to stratum corneum thickening in human skin equivalent models. Br J Dermatol 2016 Jul 21 [PMID: 27442805]
Hartmann-Fritsch F, Biedermann T, Braziulis E et al. A new model for preclinical testing of dermal substitutes for human skin reconstruction. Pediatr Surg Int 2013 Feb 1 [PMID: 23371301] (IHC-P, ICC/IF, Human, Rat) IHC-P, ICC/IF Human, Rat
Gannon OM, Merida de Long L, Endo-Munoz L et al. Dysregulation of the Repressive H3K27 Trimethylation Mark in Head and Neck Squamous Cell Carcinoma Contributes to Dysregulated Squamous Differentiation. Clin Cancer Res 2013 Jan 15 [PMID: 23186778] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Hartmann-Fritsch F, Hosper N, Luginbuhl J et al. Human amniotic fluid derived cells can competently substitute dermal fibroblasts in a tissue-engineered dermo-epidermal skin analog Pediatr Surg Int 2012 Nov 9 [PMID: 23138462] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Braziulis E, Diezi M, Biedermann T et al. Modified Plastic Compression of Collagen Hydrogels Provides an Ideal Matrix for Clinically Applicable Skin Substitutes. Tissue Eng Part C Methods. 2012 Feb 2. [PMID: 22195768] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Muller FB, Huber M, Kinaciyan T, Hausser I, Schaffrath C, Krieg T, Hohl D, Korge BP, Arin MJ. A human keratin 10 knockout causes recessive epidermolytic hyperkeratosis. Hum Mol Genet;15(7):1133-41. 2006 Apr 1. [PMID: 16505000] (ICC/IF) ICC/IF
Boelsma E, Gibbs S, Faller C, Ponec M. Characterization and comparison of reconstructed skin models: morphological and immunohistochemical evaluation. Acta Derm Venereol;80(2):82-8. 2000 Mar-Apr. [PMID: 10877124]
Machesney M, Tidman N, Waseem A, Kirby L, Leigh I. Activated keratinocytes in the epidermis of hypertrophic scars. Am J Pathol;152(5):1133-41. 1998 May. [PMID: 9588880]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 1 Antibody (NB100-2756). (Showing 1 - 1 of 1 FAQs).

  1. For NB100-2756 would you be able to give more information on the immunogen?
    • The immunogen for this product can be found in the Tail region of the Cytokeratin 1 protein between amino acids 600-644.uniprot site

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2756AF405
Alexa Fluor 488 Labeled NB100-2756AF488
Alexa Fluor 647 Labeled NB100-2756AF647
Alexa Fluor 700 Labeled NB100-2756AF700
Biotin Labeled NB100-2756B
DyLight 350 Labeled NB100-2756UV
DyLight 405 Labeled NB100-2756V
DyLight 488 Labeled NB100-2756G
DyLight 550 Labeled NB100-2756R
DyLight 650 Labeled NB100-2756C
DyLight 680 Labeled NB100-2756FR
DyLight 755 Labeled NB100-2756IR
FITC Labeled NB100-2756F
HRP Labeled NB100-2756H

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Bioinformatics

Gene Symbol KRT1
Entrez
Uniprot