Cytokeratin 1 Antibody (LHK1) - BSA Free

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a ...read more
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a ...read more
Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Cytokeratin 1 antibody (LHK1) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
LHK1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Cytokeratin 1 Antibody (LHK1) - BSA Free Summary

Immunogen
C-terminal peptide sequence of human Cytokeratin 1 [UniProt# P04264]
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2-10 ug/ml
  • Immunohistochemistry-Frozen 5-10 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Western Blot 0.5-2 ug/ml
Application Notes
This Cytokeratin 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Immunohistochemistry-paraffin sections and Western blot.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-2756 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cytokeratin 1 Antibody (LHK1) - BSA Free

  • CK1
  • CK-1
  • cytokeratin 1
  • Cytokeratin-1
  • cytokeratin-1,67 kDa cytokeratin
  • EHK
  • EHK1
  • epidermolytic hyperkeratosis 1
  • EPPK
  • Hair alpha protein
  • K1
  • keratin 1
  • keratin, type II cytoskeletal 1
  • Keratin-1
  • KRT1A
  • KRTA
  • NEPPK
  • Type-II keratin Kb1

Background

Cytokeratin 1 is a heterotetramer of two type I as well as two type II keratins localized in the cell membrane and is generally associated with keratin-10 (KRT10). It has been suggested to regulate the activity of kinases such as PKC/SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). Cytokeratin 1 and its heterodimer partner KRT10 are the major constituents of 4 intermediate filament cytoskeleton in suprabasal epidermis/keratinocytes, and their upregulation precedes expression of filaggrin and cornified envelope proteins. Covalent crosslinking of cytokeratin 1 but not of KRT10 to these proteins, in addition to a complex series of lipids, ultimately forms the cornified/lipid envelope, that constitutes the epidermal barrier together with Langerhans cells. Defects in cytokeratin 1 have been linked to epidermolytic hyperkeratosis (EHK), ichthyosis hystrix Curth-Macklin type (IHCM), palmoplantar keratoderma non-epidermolytic (NEPPK), ichthyosis annular epidermolytic (AEI), palmoplantar keratoderma striate type 3 (SPPK3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2756
Species: Hu, Rt
Applications: WB, ICC/IF, IHC

Publications for Cytokeratin 1 Antibody (NB100-2756)(15)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-P.


Filter By Application
ICC/IF
(6)
IF/IHC
(5)
IHC-P
(3)
All Applications
Filter By Species
Human
(9)
Rat
(2)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-2756 Applications Species
Iljas J, J. R, J.A. M et al. A human skin equivalent burn model to study the effect of a nanocrystalline silver dressing on wound healing Burns 2020-07-01 [PMID: 32830005] (IHC-P, Human) IHC-P Human
Li X, Tang M, Zhu Q et al. The exosomal integrin alpha5beta1/AEP complex derived from epithelial ovarian cancer cells promotes peritoneal metastasis through regulating mesothelial cell proliferation and migration Cell Oncol (Dordr) 2020-02-21 [PMID: 32080801] (IF/IHC, Human) IF/IHC Human
Arita S, Hatta M, Uchida K et al. Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats. J. Periodont. Res. 2018-04-23 [PMID: 29687476] (Rat) Rat
Kim HJ, Lee E, Lee M et al. Phosphodiesterase 4B plays a role in benzophenone-3-induced phototoxicity in normal human keratinocytes Toxicol. Appl. Pharmacol. 2017-11-25 [PMID: 29183759] (IF/IHC, Human) IF/IHC Human
Ha D, Bing SJ, Ahn G et al. Blocking Glutamate Carboxypeptidase II Inhibits Glutamate Excitotoxicity and Regulates Immune Responses in Experimental Autoimmune Encephalomyelitis FEBS J. 2016-07-22 [PMID: 27444540] (IF/IHC, Human) IF/IHC Human
McGovern JA, Meinert C, de Veer SJ et al. Attenuated kallikrein-related peptidase activity disrupts desquamation and leads to stratum corneum thickening in human skin equivalent models. Br J Dermatol 2016-07-21 [PMID: 27442805]
Hartmann-Fritsch F, Biedermann T, Braziulis E et al. A new model for preclinical testing of dermal substitutes for human skin reconstruction. Pediatr Surg Int 2013-02-01 [PMID: 23371301] (IHC-P, ICC/IF, Human, Rat) IHC-P, ICC/IF Human, Rat
Gannon OM, Merida de Long L, Endo-Munoz L et al. Dysregulation of the Repressive H3K27 Trimethylation Mark in Head and Neck Squamous Cell Carcinoma Contributes to Dysregulated Squamous Differentiation. Clin Cancer Res 2013-01-15 [PMID: 23186778] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Hartmann-Fritsch F, Hosper N, Luginbuhl J et al. Human amniotic fluid derived cells can competently substitute dermal fibroblasts in a tissue-engineered dermo-epidermal skin analog Pediatr Surg Int 2012-11-09 [PMID: 23138462] (IF/IHC, ICC/IF, Human) IF/IHC, ICC/IF Human
Braziulis E, Diezi M, Biedermann T et al. Modified Plastic Compression of Collagen Hydrogels Provides an Ideal Matrix for Clinically Applicable Skin Substitutes. Tissue Eng Part C Methods. 2012-02-02 [PMID: 22195768] (IF/IHC, ICC/IF, Human) IF/IHC, ICC/IF Human
Show All 15 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 1 Antibody (NB100-2756). (Showing 1 - 1 of 1 FAQs).

  1. For NB100-2756 would you be able to give more information on the immunogen?
    • The immunogen for this product can be found in the Tail region of the Cytokeratin 1 protein between amino acids 600-644.uniprot site

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Bioinformatics

Gene Symbol KRT1
Entrez
Uniprot