![Immunocytochemistry/Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-48877] - Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48877-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-48877] - Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Cytochrome b5 type A Antibody - BSA Free (NBP2-48877) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: HSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CYB5A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome b5 type A Antibody (NBP2-48877)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CYB5A |
![Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-48877] - Staining in human liver and skeletal muscle tissues using anti-CYB5A antibody. Corresponding CYB5A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-Immunohistochemistry-Paraffin-NBP2-48877-img0007.jpg "Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-48877] - Staining in human liver and skeletal muscle tissues using anti-CYB5A antibody. Corresponding CYB5A RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-48877] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-Immunohistochemistry-Paraffin-NBP2-48877-img0005.jpg "Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-48877] - Staining of human liver shows high expression.")
![Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-48877] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Cytochrome-b5-type-A-Antibody-Immunohistochemistry-Paraffin-NBP2-48877-img0006.jpg "Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-48877] - Staining of human skeletal muscle shows low expression as expected.")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")