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Home » Cytochrome b5 Outer Mitochondrial Membrane » Cytochrome b5 Outer Mitochondrial Membrane Antibodies » Cytochrome b5 Outer Mitochondrial Membrane Antibody

Cytochrome b5 Outer Mitochondrial Membrane Antibody

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Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP2-92276] - Analysis of extracts of various cell lines, using Cytochrome b5 Outer Mitochondrial Membrane at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP2-92276] - Analysis of extracts of various cell lines, using Cytochrome b5 Outer Mitochondrial Membrane at 1:1000 dilution.Secondary antibody: HRP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Cytochrome b5 Outer Mitochondrial Membrane Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 16-150 of human CYB5B (NP_085056.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYB5B
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Cytochrome b5 Outer Mitochondrial Membrane Antibody

  • CYB5M
  • CYB5-M
  • CYPB5M
  • Cytochrome b5 outer mitochondrial membrane isoform
  • cytochrome b5 type B (outer mitochondrial membrane)
  • cytochrome b5 type B
  • DKFZp686M0619
  • OMB5
  • outer mitochondrial membrane cytochrome b5
  • type 2 cyt-b5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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A Antibody
NBP2-49284
Immunocytochemistry/Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-49284] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-49284] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: Cytochrome b5 type A Antibody [NBP2-49284] - Staining of human skeletal muscle shows low expression as expected.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
SYNJ2BP/ARIP2
Antibody
NBP1-88985
Immunocytochemistry/Immunofluorescence: SYNJ2BP/ARIP2 Antibody [NBP1-88985] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: SYNJ2BP/ARIP2 Antibody [NBP1-88985] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SYNJ2BP/ARIP2 Antibody [NBP1-88985] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in distal tubules.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Apolipoprotein
E/ApoE Antibody (WUE- ...
NB110-60531
Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
(2 Reviews)
(28 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Cytochrome P450
17A1 Antibody (OTI3F1
NBP2-01151
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
(2 Reviews)
(3 Publications)
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Applications: Flow, IHC, IHC-P, WB
CYB5R3 Antibody
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NBP2-02705
Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - Analysis of Jurkat cells, using anti-CYB5R3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.
Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.
Species: Ca, Hu, Pm, Pm
Applications: Flow, IHC, IHC-P, WB
HSD3B1 Antibody
(FDO66Q)
NB110-78644
Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.
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Species: Pm, Bv, Gt, Hu, Mu, Po, Pm, Rt, Sh
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VDAC2 Antibody
NBP2-20849
Western Blot: VDAC2 Antibody [NBP2-20849] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term diluted at 1:1000.
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Paraffin-embedded rat kidney. VDAC2 antibody [C2C3], C-term diluted at 1:500.
Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Paraffin-embedded mouse intestine. VDAC2 antibody [C2C3], C-term diluted at 1:500
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Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
MOSC2 Antibody
NBP1-82167
Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining in human parathyroid gland and lymph node tissues using anti-MARC2 antibody. Corresponding MARC2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human cerebral cortex, kidney, lymph node and parathyroid gland using Anti-MARC2 antibody NBP1-82167 (A) shows similar protein distribution across tissues to independent antibody NBP1-82168 (B).
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Cytochrome c
Antibody (7H8.2C12) - ...
NB100-56503
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY licence.
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Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
Calretinin Antibody
NBP1-88220
Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-88220] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Western Blot: Calretinin Antibody [NBP1-88220] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in granular layer.
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MOCOS Antibody
NBP2-14243
Western Blot: MOCOS Antibody [NBP2-14243] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Simple Western: MOCOS Antibody [NBP2-14243] - Simple Western lane view shows a specific band for MOCOS in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: MOCOS Antibody [NBP2-14243] - Electropherogram image(s) of corresponding Simple Western lane view. MOCOS antibody was used at 1:30 dilution on RT-4 lysate(s).
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
POR/Cytochrome
P450 Reductase Antibo ...
NBP1-47914
Flow Cytometry: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 Reductase antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of HT29 cells using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 Reductase.
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CYP11A1 Antibody
NBP1-85368
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.
(4 Reviews)
(2 Publications)
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Applications: ICC/IF, IHC, IHC-P, WB
CD320/TCblR/8D6A
Antibody
AF1557
CD320/TCblR/8D6A Antibody
(2 Reviews)
(1 Publication)
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PORCN Antibody
NBP1-59677
Western Blot: PORCN Antibody [NBP1-59677] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.
Western Blot: PORCN Antibody [NBP1-59677] - Sample Tissue: Human Hela Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: PORCN Antibody [NBP1-59677] - SampleType: Mouse Uterus - Dilution 1 in 300. - secondary antibody dilution 1 in 1000.
(1 Publication)
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Applications: IHC, IHC-P, WB
Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.
Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.
Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
(9 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MOSC1 Antibody
NBP1-82122
Western Blot: MOSC1 Antibody [NBP1-82122] - Analysis in human cell line BEWO.
Immunocytochemistry/Immunofluorescence: MOSC1 Antibody [NBP1-82122] - Staining of human cell line A-431 shows localization to mitochondria.
Species: Hu
Applications: ICC/IF, WB
Cytochrome b5
Outer Mitochondrial M ...
NBP2-92276
Cytochrome b5 Outer Mitochondrial Membrane Antibody
Species: Hu, Mu
Applications: WB

Publications for Cytochrome b5 Outer Mitochondrial Membrane Antibody (NBP2-92276) (0)

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Bioinformatics

Gene Symbol CYB5B