MOSC2 Antibody - BSA Free

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Analysis in human parathyroid gland and lymph node tissues using NBP1-82167 antibody. Corresponding MARC2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human cerebral cortex, kidney, lymph node and parathyroid gland using Anti-MARC2 antibody NBP1-82167 (A) shows similar protein distribution across tissues to independent antibody NBP1-82168 (B).

Western Blot: MOSC2 Antibody [NBP1-82167] - Analysis in human cell line TD47D.

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human lymph node shows low expression as expected.

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human lymph node shows very weak granular cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: MOSC2 Antibody [NBP1-82167] - Staining of human parathyroid gland shows strong granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MOSC2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MOSC2 Antibody - BSA Free (NBP1-82167) is a polyclonal antibody validated for use in IHC and WB. Anti-MOSC2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FQVAYPDYCPLLIMTDASLVDLNTRMEKKMKMENFRPNIVVTGCDAFEEDTWDELLIGSVEVKKVMACPRCILTTVDPDTGVIDRKQPLDTLKSYRL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 25144769). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  MOSC2 Recombinant Protein Antigen (NBP1-82167PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25144769).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MOSC2 Antibody - BSA Free

• FLJ20605
• MOCO sulphurase C-terminal domain containing 2
• MOSC domain-containing protein 2, mitochondrial
• RP11-270A6.1

Background

Catalytic component of the benzamidoxime prodrug-converting complex, a complex required to reduce N-hydroxylated structures, such as benzamidoxime prodrug. Benzamidoxime is an amidine prodrug produced by N-hydroxylation which is used to enhance bioavailability and increase intestinal absorption. It is then reduced into benzamidine, its active amidine, by the benzamidoxime prodrug-converting complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MOSC2 Antibody (NBP1-82167)(4)

Publications using NBP1-82167

• Neve EP, Kofeler H, Hendriks DF et al. Expression and Function of mARC: Roles in Lipogenesis and Metabolic Activation of Ximelagatran. PLoS One 2015-01-01 [PMID: 26378779] (WB, Human)
• Thinon E, Serwa RA, Broncel M et al. Global profiling of co- and post-translationally N-myristoylated proteomes in human cells. Nat Commun 2014-09-26 [PMID: 25255805] (WB, Human)
• Jakobs HH, Mikula M, Havemeyer A et al. The N-Reductive System Composed of Mitochondrial Amidoxime Reducing Component (mARC), Cytochrome b5 (CYB5B) and Cytochrome b5 Reductase (CYB5R) Is Regulated by Fasting and High Fat Diet in Mice. PLoS One 2014-01-01 [PMID: 25144769] (WB, Mouse)
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: MARC2