Cystatin C Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Cystatin C Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C
  Ala21-Ala140
  Accession # P21460
• Specificity: Detects mouse Cystatin C in ELISAs and Western blots. In sandwich ELISAs, approximately 30% cross-reactivity with recombinant rat Cystatin C is observed, less than 0.2% cross-reactivity with recombinant human (rh) Cystatin C is observed and less than 0.4% cross-reactivity with recombinant mouse (rm) Cathepsin B, rmActive Cathepsin C (2336-CY), rmCathepsin C (1034-CY), rmCathepsin D, rmCathepsin E, rmCathepsin H, rmCathepsin L, rmCathepsin X/Z/P, rmCystatin B, rmCystatin E/M and rmCystatin F is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CST3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture:Mouse Cystatin C Antibody (Catalog # AF1238) ELISA Detection: Mouse Cystatin C Biotinylated Antibody (Catalog # BAF1238) Standard: Recombinant Mouse Cystatin C (Catalog # 1238-PI)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin C Antibody [Biotin]

• ARMD11
• bA218C14.4 (cystatin C)
• CST3
• cystatin 3
• cystatin C (amyloid angiopathy and cerebral hemorrhage)
• Cystatin C
• cystatin-3
• cystatin-C
• Gamma-trace
• MGC117328
• Neuroendocrine basic polypeptide
• Post-gamma-globulin

Background

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).

1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
4. Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CST3
• Uniprot:
  • P21460()