Cystatin A Antibody (224705) [Unconjugated]

Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Cystatin A Monoclonal Antibody (Catalog # MAB1407) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cystatin A at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 224705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cystatin A Antibody (224705) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Cystatin A
  Ile2-Phe98
  Accession # P01040
• Specificity: Detects human Cystatin A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatins B, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSTA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin A Antibody (224705) [Unconjugated]

• AREI
• CSTA
• cystatin A (stefin A)
• Cystatin A
• cystatin AS
• cystatin-A
• Stefin A
• STF1
• STF1Cystatin-AS
• STFA
• STFAStefin-A

Background

Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).

1. Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
2. Abrahamson, M. (1994) Methods Enzymol. 244:685.
3. Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
4. Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
5. Sinha, A.A. et al. (2002) Cancer 94:3141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin A Antibody (MAB1407)(1)

Publication using MAB1407

• Feldman AS, Banyard J, Wu CL, McDougal WS, Zetter BR Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression. Clin. Cancer Res., 2009-02-01;15(3):1024-31. 2009-02-01 [PMID: 19188175] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CSTA
• Uniprot:
  • P01040()