Cyr61/CCN1 Antibody

Images

 
Biological Strategies: Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Total protein from Human liver, spleen and HepG2 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody [NB100-356] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.
Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-356. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.
Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.
Simple Western: Cyr61/CCN1 Antibody [NB100-356] - Simple Western lane view shows a specific band for CYR61 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Cyr61/CCN1 Antibody Summary

Immunogen
A synthetic peptide made to the human CYR61 protein sequence (between residues 150-250). [UniProt# O00622]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYR61
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Simple Western 1:400
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Block/Neutralize
Application Notes
This CYR61 antibody is useful for Blocking/Neutralizing, Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin embedded sections. Immunohistochemistry was reported in scientific literature. In Western blot a band is observed at approx. 37 kDa, representing the processed form of CYR61 protein. Antigen retrieval was not used on the IHC-P pictures below. IF/ICC (PMID 18202125), Blocking/Neutralizing (PMID 22282654).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Artery Lysate (NB820-59344)
Cyr61/CCN1 Lysate (NBL1-09705)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-356 in the following applications:

Publications
Read Publications using
NB100-356 in the following applications:

Reactivity Notes

The immunogen sequence has 88% homology to rat and mouse. Rabbit reactivity reported in scientific literature (PMID: 22401280)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyr61/CCN1 Antibody

  • CCN family member 1
  • CCN1
  • CCN1IGF-binding protein 10
  • Cyr61
  • Cysteine-rich angiogenic inducer 61
  • cysteine-rich, angiogenic inducer, 61
  • GIG1
  • GIG1cysteine-rich heparin-binding protein 61
  • IBP-10
  • IGFBP-10
  • IGFBP10cysteine-rich, anigogenic inducer, 61
  • Insulin-like growth factor-binding protein 10
  • protein CYR61
  • Protein GIG1

Background

CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyr61/CCN1 Antibody (NB100-356)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-P
(3)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-356 Applications Species
Li Y, Adur MK, Kannan A et al. Progesterone Alleviates Endometriosis via Inhibition of Uterine Cell Proliferation, Inflammation and Angiogenesis in an Immunocompetent Mouse Model. PLoS One 2016 Oct 24 [PMID: 27776183] (Mouse) Mouse
Klein R, Stiller S, Gashaw I. Epidermal growth factor upregulates endometrial CYR61 expression via activation of the JAK2/STAT3 pathway Reprod Fertil Dev. 2012 [PMID: 22401280] (ICC/IF, Rabbit) ICC/IF Rabbit
Haseley A, Boone S, Wojton J et al. Extracellular matrix protein CCN1 limits oncolytic efficacy in glioma. Cancer Research. 2012 Jan 26. [PMID: 22282654]
Xie JJ, Xu LY, Wu JY et al. Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells. Am J Pathol. 2010 Feb [PMID: 20056838] (WB, IHC-P, Human) WB, IHC-P Human
Dobroff AS, Wang H, Melnikova VO et al. Silencing cAMP-response element-binding protein (CREB) identifies CYR61 as a tumor suppressor gene in melanoma. J Biol Chem. 2009 Sep [PMID: 19632997] (IHC-P, Human) IHC-P Human
Xie JJ, Xu LY, Xie YM. Roles of ezrin in the growth and invasiveness of esophageal squamous carcinoma cells. Int J Cancer. 2009 Jun [PMID: 19165868] (WB, IHC-P, Human) WB, IHC-P Human
Gashaw I, Stiller S, Boing C, Kimmig R, Winterhager E. Premenstrual Regulation of the Pro-Angiogenic Factor CYR61 in Human Endometrium. Endocrinology;149(5):2261-9. May 1 2008. [PMID: 18202125] (ICC/IF, IHC) ICC/IF, IHC

Reviews for Cyr61/CCN1 Antibody (NB100-356) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB100-356:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Other 12/12/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22401280
SpeciesOther

Blocking

Blocking DetailsSee PMID 22401280

Details

Detection NotesSee PMID 22401280

Comments

CommentsPublished in PMID: 22401280
Western Blot Cyr61/CCN1 NB100-356
Enlarge
reviewed by:
chandra kurapaty
WB Human 06/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedMDA MB 231 whole cell lysates and conditioned medium
SpeciesHuman
Lot071103

Blocking

Blocking Details5% milk in TBST 1h RT or 4C O/N

Primary Anitbody

Dilution Ratio1:1000 in 5% milk in TBST, 3h RT or 4C O/N

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#cell signalling 7074
Secondary Concentration1:5000

Details

Detection NotesECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyr61/CCN1 Antibody (NB100-356). (Showing 1 - 2 of 2 FAQs).

  1. What does the following sentence in the datasheet mean? "Blocking/Neutralizing 50-200 molar excess"
    • NB100-356 has been validated for blocking/neutralizing experiment and in order to do this experiment you need to have the molar concentration of the actual protein in the sample and we recommend using 50-200 molar excess (more than the protein). You can calculate how much of the antibody needs to be added for neutralizing experiment considering NB100-356 is a rabbit IgG and the molecular weight is approximately 160kDa = 160,000 g/mol and based on the molar concentration of the protein, add 50-200 times the amount of the antibody to the samples.
  2. I am looking for an antibody that detects human Cyr61 but does not react with mouse Cyr61. Do you have any data to suggest that antibody NB100-356 might work for my experiments?
    • For product NB100-356, we have unfortunately not tested for cross reactivity with the mouse protein. We therefore, would not be able to guarantee that the antibody would not detect the mouse protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-356AF405
Alexa Fluor 488 Labeled NB100-356AF488
Alexa Fluor 647 Labeled NB100-356AF647
Alexa Fluor 700 Labeled NB100-356AF700
Biotin Labeled NB100-356B
DyLight 405 Labeled NB100-356V
DyLight 488 Labeled NB100-356G
DyLight 550 Labeled NB100-356R
DyLight 650 Labeled NB100-356C
FITC Labeled NB100-356F
HRP Labeled NB100-356H

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Anonymous
12/12/2014
Application: ICC
Species: Other

chandra kurapaty
06/18/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CYR61
Entrez
Uniprot