CYP46A1 Antibody (N8-P6E4*H8) - BSA Free

Western Blot: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.

Immunohistochemistry: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - Shows strong cytoplasmic staining in metastatic colorectal cancer (A) and primary colorectal (B) compared to normal colon mucosa (C).

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: N8-P6E4*H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CYP46A1 Antibody (N8-P6E4*H8) - BSA Free Summary

• Description: The clone number is listed as N8-P6E4*H8 in the scientific literature.
• Immunogen: Ovalbumin-conjugated synthetic peptide- PVLCTLRPR (C-terminal sequence)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP46A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s):WB - overexpression lysates
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CYP46A1 Antibody (N8-P6E4*H8) - BSA Free

• CH24H
• cholesterol 24-hydroxylase
• CYP46CP46
• Cytochrome P450 46A1
• cytochrome P450, family 46, subfamily A, polypeptide 1
• cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
• EC 1.14.13.98

Background

CYP46A1 (cytochrome P450, family 46, subfamily A, polypeptide 1) belongs to the cytochrome P450 family which consist of a variety of enzymes that are involved in catalyzing reactions. CYP46A1 is expressed in the brain and is involved in turnover of cholesterol through the conversion of cholesterol to 24S-hydroxycholesterol. CYP46A1 is known to have interactions with CH25H, CYP27A1, CYP39A1, CYP7A1 and CEL. Recent studies of CYP46A1 have been conducted in relation to Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP46A1