CYP27A1 Antibody

Images

 
Western Blot: CYP27A1 Antibody [NBP2-16063] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: CYP27A1 Antibody [NBP2-16063] - Immunohistochemical analysis of paraffin-embedded hela xenograft, using antibody at 1:500 dilution.
Western Blot: CYP27A1 Antibody [NBP2-16063] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Order Details

CYP27A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CYP27A1. The exact sequence is proprietary.
Localization
Mitochondrion membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP27A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Porcine (80%), Rabbit (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CYP27A1 Antibody

  • 12-alpha-triol 27-hydroxylase
  • 7-alpha
  • cholestanetriol 26-monooxygenase
  • CP27,5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase
  • CTX5-beta-cholestane-3-alpha
  • CYP275-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase
  • Cytochrome P450 27
  • cytochrome P450, family 27, subfamily A, polypeptide 1
  • cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinousxanthomatosis), polypeptide 1
  • Cytochrome P-450C27/25
  • EC 1.14.13.15
  • sterol 26-hydroxylase, mitochondrial
  • Sterol 27-hydroxylase
  • Vitamin D(3) 25-hydroxylase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYP27A1
Uniprot