CYP11B2 Overexpression Lysate

Images

 
Western Blot: CYP11B2 Overexpression Lysate (Adult Normal) [NBL1-09672] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP11B2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CYP11B2 Overexpression Lysate Summary

Description
Transient overexpression lysate of cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein The lysate was created in HEK293T cells, using Plasmid ID RC215476 and based on accession number NM_000498. The protein contains a C-MYC/DDK Tag.
Gene
CYP11B2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
54.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CYP11B2 Overexpression Lysate

  • ALDOSCYP11B
  • Aldosterone synthase
  • Aldosterone-synthesizing enzyme
  • CPN2
  • CYP11BL
  • CYPXIB2
  • cytochrome P450 11B2, mitochondrial
  • cytochrome P450, family 11, subfamily B, polypeptide 2
  • cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2
  • Cytochrome P-450Aldo
  • Cytochrome P-450C18
  • EC 1.14.15
  • EC 1.14.15.4
  • EC 1.14.15.5
  • mitochondrial cytochrome P450, family 11, subfamily B, polypeptide 2
  • P450aldo
  • P450C18
  • P-450C18
  • steroid 11-beta/18-hydroxylase
  • steroid 11-beta-monooxygenase
  • Steroid 18-hydroxylase
  • steroid 18-hydroxylase, aldosterone synthase, P450C18, P450aldo

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-68883
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NB100-62346
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP2-45552
Species: Hu
Applications: WB, IHC
NB100-1533
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP1-52823
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-562
Species: Hu, Mu, Rt, Ch, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, B/N
NBP2-75461
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-85368
Species: Hu, RM
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77078
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-13893
Species: Hu
Applications: WB, IHC, IHC-P
NB100-1596
Species: Hu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-89200
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
AF7895
Species: Hu
Applications: WB, IHC
NBP1-85466
Species: Hu, Mu, Fe
Applications: WB, IHC, IHC-P
NBP1-33485
Species: Hu, Mu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37898
Species: Hu
Applications: WB, IHC, IHC-P
AF2156
Species: Hu, Mu
Applications: WB, IHC
NBL1-09672
Species: Hu
Applications: WB

Publications for CYP11B2 Lysate (NBL1-09672) (0)

There are no publications for CYP11B2 Lysate (NBL1-09672).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CYP11B2 Lysate (NBL1-09672) (0)

There are no reviews for CYP11B2 Lysate (NBL1-09672). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CYP11B2 Lysate (NBL1-09672) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-09672

Additional CYP11B2 Products

Bioinformatics Tool for CYP11B2 Lysate (NBL1-09672)

Discover related pathways, diseases and genes to CYP11B2 Lysate (NBL1-09672). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CYP11B2 Lysate (NBL1-09672)

Discover more about diseases related to CYP11B2 Lysate (NBL1-09672).
 

Pathways for CYP11B2 Lysate (NBL1-09672)

View related products by pathway.

PTMs for CYP11B2 Lysate (NBL1-09672)

Learn more about PTMs related to CYP11B2 Lysate (NBL1-09672).
 

Research Areas for CYP11B2 Lysate (NBL1-09672)

Find related products by research area.

Blogs on CYP11B2

There are no specific blogs for CYP11B2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CYP11B2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol CYP11B2