Cyclin T1 Antibody (SR2399)

Western Blot: Cyclin T1 Antibody (SR2399) [NBP3-27694] - Western blot analysis of Cyclin T1 in Hela, Jurkat, K562 lysates

Western Blot: Cyclin T1 Antibody (SR2399) [NBP3-27694] - Western blot analysis of Cyclin T1 in mouse brain, rat brain lysates

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: SR2399
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cyclin T1 Antibody (SR2399) Summary

• Description: Novus Biologicals Rabbit Cyclin T1 Antibody (SR2399) (NBP3-27694) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human Cyclin T1 (Uniprot #: O60563)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCNT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunoprecipitation 1:20
  • Western Blot 1:500-1:1000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cyclin T1 Antibody (SR2399)

• CCNT
• CCNT1
• CCNTcyclin-T
• CDK9-associated C-type protein
• cyclin C-related protein
• cyclin T1
• cyclin T1b
• Cyclin-T
• cyclin-T1
• CYCT1
• HIVE1
• human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
• Human immunodeficiency virus-1 expression
• subunit of positive elongation transcription factor b

Background

Cyclin T1 belongs to the cyclin family and is a primary regulator of CDK kinases. More specifically, this cyclin associates with CDK9 complex, also known as transcription elongation factor B (P-TEFb). This complex allows for the transcription of viral genes and is also involved in the regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. Cyclin interacts with HIV-1, HIV-2, Tat and has a high affinity to TAR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNT1