Cyclin D1 Antibody (DCS-6)

Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Human uterine mesodermal tumor cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Cyclin D1 (DCS-6). The antibody was developed with Anti-Mouse IgG (Fab specific), Alkaline Phosphatase conjugate and a NBT/BCIP substrate.

Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Antibody (DCS-6).

Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (DCS-6).

Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-paraffin human mantle cell lymphoma stained with Cyclin D1 Ab (Clone DCS-6). Note nuclear staining of tumor cells.

Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-15189. Cyclin D1 staining of HCT116 cells using Alexa Fluor 647 conjugated Cyclin D1 antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: DCS-6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cyclin D1 Antibody (DCS-6) Summary

• Immunogen: Human recombinant full length Cyclin D1 protein was used as immunogen to generate this antibody.
• Localization: Nuclear
• Marker: G1-Cyclin & Mantle Cell Marker
• Specificity: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCND1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
• Application Notes: Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min. Use in Immunohistochemistry reported in scientific literature (PMID: 30050877). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Control:

  Cyclin D1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for Cyclin D1 Antibody (DCS-6)

• B-cell lymphoma 1 protein
• BCL-1 oncogene
• Bcl-1
• BCL1D11S287E
• CCND1
• cyclin D1 (PRAD1: parathyroid adenomatosis 1)
• Cyclin D1
• Cyl-1
• G1/S-specific cyclin D1
• G1/S-specific cyclin-D1
• PRAD1 oncogene
• PRAD1
• PRAD1B-cell CLL/lymphoma 1
• U21B31

Background

Cyclin D1 is a member of a superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating CDKs. The D-type subfamily includes cyclin D1, D2, and D3. All three are differentially, and sometimes redundantly, expressed in different lineages. However, in every cell type at least one of the three can be detected. Cyclin D1 as well as D2 and D3 have central roles in linking exogenous growth regulating stimuli with the cycling machinery of cells. They are typically considered to be G1 cyclins; synthesis is initiated during G1 and their expression drives the G1/S phase transition.
The deregulation of cyclin D1 or other D types is commonly involved in a wide range of cancers. For example cyclin D1 is activated by chromosomal rearrangements in both parathyroid adenomas and B-cell neoplasms. The DCS-6 antibody is specific for cyclin D1, and has been key in elucidating the function and involvement of cyclin D1 in oncogenesis. Results with this antibody have help to show that Cyclin D1 is over expressed in many different kinds of cancer. For example, about 50-70% of mantle cell lymphomas and 40% of breast carcinomas are cyclin D1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin D1 Antibody (NBP2-15189)(1)

Publication using NBP2-15189

• Russo I, Carrizzo A, Bochicchio S et al. siRNA Delivery for Control of Cyclin D1 and E2F1 Expression in Crohn's Disease. Transl Med UniSa Jul 1 2017 [PMID: 30050877] (IHC, Human)

Bioinformatics

• Gene Symbol: CCND1
• Uniprot:
  • P24385()