Cyclin B1 Antibody (CCNB1/1098) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
CCNB1/1098
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Cyclin B1 Antibody (CCNB1/1098) - Azide and BSA Free Summary

Immunogen
Recombinant human full-length CCNB1 protein
Localization
Cytoplasmic & Nuclear
Specificity
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCNB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml
  • CyTOF-ready
  • Immunofluorescence : 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 5q13.2 Molecular weight of antigen: 55-62kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Cyclin B1 Antibody (CCNB1/1098) - Azide and BSA Free

  • CCNB
  • CCNB1
  • Cyclin B1
  • G2/mitotic-specific cyclin B1
  • G2/mitotic-specific cyclin-B1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CCNB1
Entrez