Cyclin B1 Antibody (CCNB1/1098)

Images

 
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Ovarian Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
CCNB1/1098
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cyclin B1 Antibody (CCNB1/1098) Summary

Immunogen
Recombinant human full-length CCNB1 protein
Localization
Cytoplasmic & Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCNB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/mL
  • Immunofluorescence 1 - 2 ug/mL
Application Notes
Hu-chromosome location: 5q13.2 Molecular weight of antigen: 55-62kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-44687 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS, 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cyclin B1 Antibody (CCNB1/1098)

  • CCNB
  • CCNB1
  • Cyclin B1
  • G2/mitotic-specific cyclin B1
  • G2/mitotic-specific cyclin-B1

Background

In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyclin B1 Antibody (NBP2-44687) (0)

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Review for Cyclin B1 Antibody (NBP2-44687) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-44687:
Filter by Applications
ELISA
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 11/09/2018
View

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman
Lot891-2P171207

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47893AF350
Alexa Fluor 405 NBP2-47893AF405
Alexa Fluor 488 NBP2-47893AF488
Alexa Fluor 532 NBP2-47893AF532
Alexa Fluor 594 NBP2-47893AF594
Alexa Fluor 647 NBP2-47893AF647
Alexa Fluor 700 NBP2-47893AF700
Alexa Fluor 750 NBP2-47893AF750
Allophycocyanin NBP2-47893APC
Biotin NBP2-47893B
DyLight 350 NBP2-47893UV
DyLight 405 NBP2-47893V
DyLight 488 NBP2-47893G
DyLight 550 NBP2-47893R
DyLight 594 NBP2-47893DL594
DyLight 650 NBP2-47893C
DyLight 680 NBP2-47893FR
DyLight 755 NBP2-47893IR
FITC NBP2-47893F
HRP NBP2-47893H
Janelia Fluor 549 NBP2-47893JF549
Janelia Fluor 646 NBP2-47893JF646
PE NBP2-47893PE
PerCP NBP2-47893PCP

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Recent Reviews

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Anonymous
11/09/2018
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol CCNB1
Uniprot