Cyclin B1 Antibody (5G6)

Western Blot: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 5G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cyclin B1 Antibody (5G6) Summary

• Description: Novus Biologicals Mouse Cyclin B1 Antibody (5G6) (NBP1-51669) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.
• Marker: Mitosis Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCNB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Cyclin B1 Antibody (5G6)

• CCNB
• CCNB1
• Cyclin B1
• G2/mitotic-specific cyclin B1
• G2/mitotic-specific cyclin-B1

Background

In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNB1