CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CXCR7/RDC-1 Antibody [NBP1-31309] - Analysis of human pancreatic cancer cell line, using CCR7 antibody at 1:50 dilution. Blue: DAPI.

Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP1-31309] - Human gastric cancer, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Boiled and unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7/RDC-1 antibody [C1C2], Internal (NBP1-31309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

• Reactivity: Hu, Mu, Rt, Ca, RM
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CXCR7/RDC-1 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR7/RDC-1. The exact sequence is proprietary.
• Localization: Cell membrane
• Predicted Species: Canine (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACKR3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Flow Cytometry Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CXCR7/RDC-1 Antibody

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

CCR7, or C-C chemokine receptor type 7, is a G protein-coupled, seven-transmembrane domain receptor protein. Originally identified as the EBV-induced gene-1 (1), CCR7 was later found to be the receptor for the chemokines CCL21 (SLC, 6Ckine, Exodus-2) (3) and CCL19 (MIP-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CXCR7/RDC-1 Antibody (NBP1-31309)(3)

Publications using NBP1-31309

• AK Rohlfing, K Kolb, M Sigle, M Ziegler, A Bild, P Münzer, J Sudmann, V Dicenta, T Harm, MC Manke, S Geue, M Kremser, M Chatterjee, C Liang, H von Eysmon, T Dandekar, D Heinzmann, M Günter, S von Ungern, M Büttcher, T Castor, S Mencl, F Langhauser, K Sies, D Ashour, MC Beker, M Lämmerhofe, SE Autenrieth, TE Schäffer, S Laufer, P Szklanna, P Maguire, M Heikenwald, KAL Müller, DM Hermann, E Kilic, R Stumm, G Ramos, C Kleinschni, O Borst, HF Langer, D Rath, M Gawaz ACKR3 regulates platelet activation and ischemia-reperfusion tissue injury Nature Communications, 2022-04-05;13(1):1823. 2022-04-05 [PMID: 35383158]
• Katsura M, Shoji F, Okamoto T et al. Correlation between CXCR4/CXCR7/CXCL12 chemokine axis expression and prognosis in lymph-node-positive lung cancer patients Cancer Sci. 2018-01-01 [PMID: 29032612] (IHC-P, Human)
• Singh RK, Lokeshwar BL. The IL-8-regulated chemokine receptor CXCR7 stimulates EGFR signaling to promote prostate cancer growth. Cancer Res 2011-05-01 [PMID: 21398406] (WB, ICC/IF, FC, Human)

Bioinformatics

• Gene Symbol: ACKR3
• Entrez:
  • 1236(Human)
• Uniprot:
  • P32248()