CXCR5 Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

CXCR5 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse CXCR5
  Met1-Pro57, Glu112-Thr126, Lys191-Phe221, and Leu283-Ile306, joined by linker peptides
  Accession # Q04683
• Specificity: Detects mouse CXCR5 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CXCR7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR5 Antibody [Alexa Fluor® 750]

• BLR1
• BLR1MDR-15
• Burkitt lymphoma receptor 1
• Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
• Burkitt lymphoma receptor 1, GTP-binding protein
• CD185 antigen
• CD185
• chemokine (C-X-C motif) receptor 5
• C-X-C chemokine receptor type 5
• CXCR5
• CXC-R5
• MDR15CXCR-5
• MGC117347
• Monocyte-derived receptor 15

Background

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.