CXCR3 Antibody (868013R) [Unconjugated]

NS0 cell line transfected with (A) rat CXCR3 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CXCR3 Monoclonal Antibody (Catalog # MAB8109R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) staining. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Flow, CyTOF-ready
• Clone: 868013R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR3 Antibody (868013R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: NS0 mouse myeloma cell line transfected with rat CXCR3
  Accession # Q9J119
• Specificity: Detects rat CXCR3 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR3 Antibody (868013R) [Unconjugated]

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

CXCR3, also known as CD183, is an approximately 41 kDa (predicted) 7-transmembrane spanning receptor for the chemokines CXCL9, CXCL10, and CXCL11. It is expressed on activated T cells, B cells, and NK cells during inflammation. It is additionally upregulated on solid tumor cells, tumor endothelium, and cancer stem cells. CXCR3 plays a role in leukocyte recruitment to sites of inflammation. It also contributes to Th1 biased responses during autoimune diseases rheumatoid arthritis, systemic erythematosis, and type 1 diabetes. Rat CXCR3 shares 86% and 96% amino acid sequence identity with human and mouse CXCR3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9J119()