CXCR3 Antibody (49801R) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human CXCR3 or (B) irrelevant transfectants and eGFP was stained with and either Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

CXCR3 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to infiltrating lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Human CXCL11/I-TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R). The ND50 is typically 0.3-1.5 µg/mL.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 49801R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR3 Antibody (49801R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR3
  Met1-Leu368
  Accession # P49682
• Specificity: Detects human CXCR3 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 0.5-25 ug/mL
  • Neutralization 0.3-1.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR3 Antibody (49801R) [Unconjugated]

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P49682()