CXCR3 Antibody (1C6)

Product Discontinued

CXCR3 Antibody (1C6) Summary

• Immunogen: A 37aa-synthetic peptide corresponding to the N-terminus of human CXCR3 conjugated to KLH.
• Localization: Cell Membrane
• Specificity: This antibody recognizes surface expressed CXCR3, and/or PHA or anti-CD3 activated human T cells. The other species are not tested.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.1-1.0 ug/ml
  • Flow Cytometry 5-10ug/ml
  • Immunohistochemistry 1-10ug/ml
  • Immunohistochemistry-Frozen 1-10ug/ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5ug/ml
  • Western Blot 0.5-2.0ug/ml
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 29544513).
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile TBS (pH 7.2) with antibody stabilizer
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCR3 Antibody (1C6)

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

Chemokine receptors are seven-transmembrane domain G-protein coupled receptors that mediate the biological activities of chemokines. Most of these receptors exhibit promiscuous binding properties whereby several different chemokines signal through the same receptor. They are named according to the chemokine subfamily they bind. There are currently six CXCspecific receptors designated CXCR1 to CXCR6. This antibody recognizes surface expressed CXCR3, as judged by staining of CXCR3 transfected cells, but not untransfected cells. The antibody also stained human T cells and T cell clones that had been activated in vitro with PHA or anti-CD3. However, the anti-CXCR3 antibody did not react with neutrophils. The CXCR3 receptor is also known as the IP-10 receptor and the Mig receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR3 Antibody (NBP1-72223)(2)

Publications using NBP1-72223

• Satrom KM, Ennis K, Sweis BM et al. Neonatal hyperglycemia induces CXCL10/CXCR3 signaling and microglial activation and impairs long-term synaptogenesis in the hippocampus and alters behavior in rats. J Neuroinflammation. 2018-03-15 [PMID: 29544513] (IHC-P)
• Qin S, Rottman JB, Myers P, Kassam N, Weinblatt M, Loetscher M, Koch AE, Moser B, Mackay CR. The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. J Clin Invest. 101(4):746-54. 1998-02-15 [PMID: 9466968]

Bioinformatics

• Gene Symbol: CXCR3
• Entrez:
  • 2833(Human)
• Uniprot:
  • P49682()