CXCR2/IL-8RB Antibody (RM0212-24D12) - Azide and BSA Free

Immunohistochemistry-Paraffin: CXCR2/IL-8 RB Antibody (RM0212-24D12) [NBP2-12238] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP2-12238

Product Discontinued

CXCR2/IL-8RB Antibody (RM0212-24D12) - Azide and BSA Free Summary

• Immunogen: Mouse recombinant CXCR2/IL-8RB
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • CyTOF-ready
  • Flow Cytometry 1:20-1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for CXCR2/IL-8RB Antibody (RM0212-24D12) - Azide and BSA Free

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors. Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases several subtypes of CXCR have been characterized. Activation of na

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CXCR2/IL-8RB Antibody (NBP2-12238). (Showing 1 - 2 of 2 FAQ).

1. I have two technical questions about your CXCR2 antibody (NBP2-12238). 1. Which ligand does the antibody block? E.g.CXCL5, CXCL1, or all ligands? 2. Can we use it for mouse samples in vivo and vitro? If so what dilution you recommend to use?
   • NBP2-12238 binds to CXCR2, not the ligand. This is how it's neutralization activity works. We do not guarantee or recommend any of our products for in vivo use and the dosage will have to be user determined.
2. I bought the CXCR2 antibody (NBP2-12238) for in vitro testing (histology, neutralizing experiments). There is no explanation in what medium the antibody can be diluted (PBS, distilled water, etc). Could you please help me with that and tell me how this antibody should be diluted?
   • You can reconstitute this antibody with 200ul of sterile PBS (final concentration 500ug/ml). The reconstituted antibody can be aliqoted and stored frozen at less than -20C for at least for six months without any detectable loss of activity. Please avoid repeated freeze-thaw cycles.

Bioinformatics

• Gene Symbol: CXCR2
• Uniprot:
  • P35343()