Recombinant Bovine CXCL8/IL-8 Protein

ELISA: Recombinant Bovine CXCL8/IL-8 Protein [NBP3-11063] - Recombinant Bovine Interleukin-8 detected using Goat anti Bovine Interleukin-8 as the capture reagent and Goat anti Bovine Interleukin-8:Biotin as the detection reagent followed by Streptavidin:HRP.

• Reactivity: Bv
• Applications: WB, ELISA
• Concentration: LYOPH

Recombinant Bovine CXCL8/IL-8 Protein Summary

• Description: Recombinant Bovine Interleukin-8 (IL-8) is a recombinant protein corresponding to aa 23-101 of native bovine IL-8.
• Preparation Method: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL8
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Theoretical MW: 9.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 10% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.

Alternate Names for Recombinant Bovine CXCL8/IL-8 Protein

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL8
• Uniprot:
  • P79255(Bovine)