Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

ELISA: CXCL8/IL-8 ELISA Kit [Biotin] [NBP1-92706] - Standard curve

• Reactivity: Pm
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum (baboon, cynomolgus)
• Standard Curve Range: 19.5 - 1,250 U/mL
• Sensitivity: 3.0 U/mL

Monkey CXCL8/IL-8 ELISA Kit (Colorimetric) Summary

• Description: The monkey IL-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-8
• Standard Curve Range: 19.5 - 1,250 U/mL
• Sensitivity: 3.0 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8.7%
• Intra-Assay: CV% < 6.3%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CXCL8

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey CXCL8/IL-8 ELISA Kit (Colorimetric)

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8. Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes. The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CXCL8/IL-8 ELISA Kit (NBP1-92706). (Showing 1 - 2 of 2 FAQ).

1. We have received an inquiry from our customer who is interested in your product, IL8 ELISA Kit (Catalog #NBP1-92706). Could you tell us the whether the product contains monkey-derived component? Is it Monkey IL-8 Standard Concentrate?
   • Yes, this kit includes Monkey IL-8 Standard Concentrate (125000 U/ml) which will be used for creating the standard curve. The standard curve range for IL8 can be created from 19.5 to 1,250 U/mL and this kit has been suggested for IL8 detection with sensitivity limits of as little as 1.5 U/mL.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CXCL8