CXCL8/IL-8 Antibody (N11) - BSA Free

Western Blot: CXCL8/IL-8 Antibody (N11) [NBP2-00453] - Immunoblot of human peripheral blood mononuclear cells either left untreated or treated with LPS and Brefeldin A for 4 hours. Lane 1, non-reduced lysate prepared from stimulated cells; lane 2, non-reduced lysate of unstimulated cells; lane 3, reduced lysate of stimulated cells; lane 4, reduced lysate prepared from unstimulated cells. Bands were visualized using Anti-Mouse IgG HRP.

Product Discontinued

CXCL8/IL-8 Antibody (N11) - BSA Free Summary

• Immunogen: The immunogen for this antibody is IL8.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1-5 ug/ml
• Application Notes: This N11 antibody has been tested by immunoblot of peripheral blood mononuclear cells stimulated with LPS for 4 hours. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. ELISA was reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CXCL8/IL-8 Antibody (N11) - BSA Free

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

The monoclonal antibody N11 reacts specifically with the 77 amino acid form of IL-8 (IL-877, IL-8(1-77), CXCL877) and does not react with the more common 72 amino acid form. Frequently referred to as "endothelial" IL-8, the 77 amino acid form is secreted in cultured leukocytes. The 72 amino acid form is the result of efficient cleavage of an N-terminal pentamer from IL-877 after secretion. IL-8 is a C-X-C chemokine with potency in neutrophil chemotaxis and activation. IL-877 has decreased chemotactic activity when compared to IL-872, but has other specific pro-apoptotic and tumor cell chemotactic activities. IL-877 has recently been shown to be the major isoform present in premature neonates due to deficiency in serum protease activity that is not seen in term neonates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL8/IL-8 Antibody (NBP2-00453)(2)

Publications using NBP2-00453

• Maheshwari A, Voitenok NN, Akalovich S, Shaik SS, Randolph DA, Sims B, Patel RP, Killingsworth CR, Fallon MB, Ohls RK. Developmental changes in circulating IL-8/CXCL8 isoforms in neonates. Cytokine;46(1):12-6. 2009-04-01 [PMID: 19232500] (ELISA)
• Nashkevich NN, Akalovich S, Louneva N, Heavner GA, Voitenok NN. A monoclonal antibody and an enzyme immunoassay for human Ala-IL-8(77). J Immunol Methods;270(1):37-51. 2002-12-01 [PMID: 12379337]

Bioinformatics

• Gene Symbol: CXCL8