CXCL8/IL-8 Antibody (6217) [Unconjugated]

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows human PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically0.08-0.4 µg/mL.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8. e Immunoblot analysis confirming reversal of mitotic apoptosis following exogenous reconstitution of IL-8 in CXCL8-depleted cells. Experiment was performed as in a, with addition of exogenous IL-8 for the last 48 h. Cells were treated with nocodazole for 8 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8. f Immunoblot analysis detecting induction of mitotic apoptosis in response to WT1, CXCL8, or WT1 and CXCL8 knockdown compared to control knockdown in U2OS cells that were arrested in mitosis using nocodazole (8 h). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8.a. i Immunoblot analysis revealing decreased mitotic apoptosis after CDC14B knockdown in USP9XWT but not in USP9XMut U2OS cells. Before lysis cells were transfected with control or CDC14B-directed siRNA, arrested in mitosis, and collected for analysis by western blot. Throughout this figure, mean and standard deviations as error bars are displayed. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cytokines regulated by RanBP1 in lung cancer. (A) Identification of secreted factors regulated by RanBP1 using cytokine arrays. A549 cells are used, and the expression of RanBP1 is suppressed using si-RNA. (B) Comparison of the gene expression of cytokines regulated by RanBP1. (C) Analysis of cytokines regulated by RanBP1 using WB. (D) Comparison of the sphere formation ability after each neutralizing antibody treatment. The p value is less than 0.0001 versus the IgG Ab value. (E) Comparison of migration and invasion ability of cells after each neutralizing antibody treatment. Experiments performed in triplicate. (F) CSC marker protein analysis in A549 after treatment with IL-18 neutralizing antibody. (G) Confirmation of the expression levels of EMT marker proteins after treatment with the IL-18 neutralizing antibody. (H) Expression analysis of RanBP1 regulated by IL-18 using neutralizing antibodies. Error bars represent mean ± SD. Triplicate samples. * p < 0.05, ** p < 0.001, versus control. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37047826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cytokines regulated by RanBP1 in lung cancer. (A) Identification of secreted factors regulated by RanBP1 using cytokine arrays. A549 cells are used, and the expression of RanBP1 is suppressed using si-RNA. (B) Comparison of the gene expression of cytokines regulated by RanBP1. (C) Analysis of cytokines regulated by RanBP1 using WB. (D) Comparison of the sphere formation ability after each neutralizing antibody treatment. The p value is less than 0.0001 versus the IgG Ab value. (E) Comparison of migration and invasion ability of cells after each neutralizing antibody treatment. Experiments performed in triplicate. (F) CSC marker protein analysis in A549 after treatment with IL-18 neutralizing antibody. (G) Confirmation of the expression levels of EMT marker proteins after treatment with the IL-18 neutralizing antibody. (H) Expression analysis of RanBP1 regulated by IL-18 using neutralizing antibodies. Error bars represent mean ± SD. Triplicate samples. * p < 0.05, ** p < 0.001, versus control. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37047826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cytokines regulated by RanBP1 in lung cancer. (A) Identification of secreted factors regulated by RanBP1 using cytokine arrays. A549 cells are used, and the expression of RanBP1 is suppressed using si-RNA. (B) Comparison of the gene expression of cytokines regulated by RanBP1. (C) Analysis of cytokines regulated by RanBP1 using WB. (D) Comparison of the sphere formation ability after each neutralizing antibody treatment. The p value is less than 0.0001 versus the IgG Ab value. (E) Comparison of migration and invasion ability of cells after each neutralizing antibody treatment. Experiments performed in triplicate. (F) CSC marker protein analysis in A549 after treatment with IL-18 neutralizing antibody. (G) Confirmation of the expression levels of EMT marker proteins after treatment with the IL-18 neutralizing antibody. (H) Expression analysis of RanBP1 regulated by IL-18 using neutralizing antibodies. Error bars represent mean ± SD. Triplicate samples. * p < 0.05, ** p < 0.001, versus control. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37047826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cytokines regulated by RanBP1 in lung cancer. (A) Identification of secreted factors regulated by RanBP1 using cytokine arrays. A549 cells are used, and the expression of RanBP1 is suppressed using si-RNA. (B) Comparison of the gene expression of cytokines regulated by RanBP1. (C) Analysis of cytokines regulated by RanBP1 using WB. (D) Comparison of the sphere formation ability after each neutralizing antibody treatment. The p value is less than 0.0001 versus the IgG Ab value. (E) Comparison of migration and invasion ability of cells after each neutralizing antibody treatment. Experiments performed in triplicate. (F) CSC marker protein analysis in A549 after treatment with IL-18 neutralizing antibody. (G) Confirmation of the expression levels of EMT marker proteins after treatment with the IL-18 neutralizing antibody. (H) Expression analysis of RanBP1 regulated by IL-18 using neutralizing antibodies. Error bars represent mean ± SD. Triplicate samples. * p < 0.05, ** p < 0.001, versus control. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37047826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Interaction between osteosarcoma and macrophages increased IL-8 production. A Cytokine arrays of 143B-Luc cells, human macrophages, and co-culture CM. B IL-8 production during the co-culture of OS cells (143B-Luc, SJSA-1) and macrophages (THP-1 Mφ, HMDMs). C IL-8 expression in macrophages stimulated with OS-CM. D IL-8 expression in OS cells stimulated with macrophage CM. E UMAP plot of OS lung metastases. F UMAP plot of Iba1, CD163, and IL-8 expression in myeloid cell clusters. G Violin plot depicting IL-8 expression in Iba1+/− or CD163+/− myeloid cell clusters. CM conditioned medium, HMDMs human monocyte-derived macrophages, OS osteosarcoma, THP-1 Mφ THP-1-derived macrophage. Data are presented as mean ± standard deviation. **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on proliferation, migration, and invasion of osteosarcoma cells.A Cell viability assay for OS cells treated with or without recombinant IL-8 (rIL-8, 10 ng/mL) using Cell Counting Kit-8. B, C Scratch and invasion assays for OS cells treated with or without co-culture CM. The scratch assay was performed for 12 h, and the invasion assay was conducted for 24 h. Scale bars represent 500 μm. D Cell viability assay for OS cells treated with or without co-culture CM and with or without anti-IL-8 antibodies (IL-8 Abs, 1 µg/mL) using Cell Counting Kit-8. E, F Scratch and invasion assays for OS cells treated with or without co-culture CM and with or without IL-8 Abs (100 ng/mL). The scratch assay was performed for 12 h, and the invasion assay was conducted for 24 h. Scale bars represent 500 μm. CM: conditioned medium, OS: osteosarcoma, ns: not significant. Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on lung metastasis. A Schematic showing the experimental design for OS tail vein injection. 143B-Luc cells were treated with DMEM or co-culture CM for 12 h prior to tail vein injection; 143B-Luc cells (1 × 106/mouse) were injected into the tail vein of 6–7 week-old BALB/c nu/nu mice. B IVIS imaging and quantification of lung metastasis 14 days after the injection of tumors pretreated with or without co-culture CM. C Hematoxylin and eosin staining of lung sections with or without co-culture CM and quantification of lung colonies. Scale bars represent 1 mm and 500 µm. D Immunohistochemistry of Ki-67 positive cells in lung colonies with or without co-culture CM and quantification of the Ki-67 labeling index. Two colonies per sample were randomly selected, and quantification was performed for 10 colonies. Ki-67-positive cells in the colonies were counted and shown as the Ki-67 labeling index. Scale bars represent 100 µm. E Schematic showing the experimental design for OS tail vein injection using anti-IL-8 antibody. F IVIS imaging and quantification of lung metastasis 14 days after the injection of tumors pretreated with co-culture CM with or without IL-8 antibodies. G Hematoxylin and eosin staining of lung sections with co-culture CM with or without IL-8 Abs and quantification of lung colonies. Scale bars represent 1 mm and 500 µm. H Immunohistochemistry of Ki-67-positive cells in lung colonies with co-culture CM with or without IL-8 Abs and quantification of Ki-67 labeling index. Scale bars represent 100 µm. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, IVIS, in vivo imaging system, OS osteosarcoma. Data are presented as mean ± standard deviation; *p < 0.05, **p < 0.01. Each group contained five animals. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on primary growth. A Schematic showing the experimental design for subcutaneous transplantation of OS cells. 143B-Luc cells (2 × 106/mouse) were subcutaneously transplanted in 6–7-week-old BALB/c nu/nu mice, and DMEM or co-culture CM was injected into the para-tumor thrice per week. B Tumor volume with or without co-culture CM measured thrice per week after tumor transplantation. C, D Tumor weight and image of the excised tumor with or without co-cultured CM 14 days after tumor transplantation. E, F Immunohistochemistry and quantification of Ki-67 and phospho-FAK in tumor sections with or without co-culture CM 14 days after tumor transplantation. Two fields of view per sample were randomly selected, and quantification was performed in 10 fields. E Ki-67-positive cells in the field were counted and indicated as Ki-67 labeling index. Scale bars represent 50 μm. G Schematic showing the experimental design for subcutaneous OS transplantation using anti-IL-8 antibodies (IL-8 Abs). H Tumor volume after pretreatment with co-culture CM with or without IL-8 Abs (10 µg/mouse) measured thrice per week after tumor transplantation. I and J Weight and image of the excised tumor pretreated with co-culture CM with or without IL-8 Abs 14 days after tumor transplantation. K, L Immunohistochemistry and quantification of Ki-67 and phospho-FAK in tumor sections with co-culture CM with or without IL-8 Abs 14 days after tumor transplantation. Scale bars represent 50 µm. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, FAK focal adhesion kinase, OS osteosarcoma. Data are presented as mean ± standard deviation; *p < 0.05, **p < 0.01. Each group contained five animals. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Interaction between osteosarcoma and macrophages increased IL-8 production. A Cytokine arrays of 143B-Luc cells, human macrophages, and co-culture CM. B IL-8 production during the co-culture of OS cells (143B-Luc, SJSA-1) and macrophages (THP-1 Mφ, HMDMs). C IL-8 expression in macrophages stimulated with OS-CM. D IL-8 expression in OS cells stimulated with macrophage CM. E UMAP plot of OS lung metastases. F UMAP plot of Iba1, CD163, and IL-8 expression in myeloid cell clusters. G Violin plot depicting IL-8 expression in Iba1+/− or CD163+/− myeloid cell clusters. CM conditioned medium, HMDMs human monocyte-derived macrophages, OS osteosarcoma, THP-1 Mφ THP-1-derived macrophage. Data are presented as mean ± standard deviation. **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
• Clone: 6217
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL8/IL-8 Antibody (6217) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-8/CXCL8
  Ser28-Ser99
  Accession # P10145
• Specificity: Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.08-0.4 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 3 ug/mL
• Application Notes: ELISA Detection: Human CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF208)
  Standard: Recombinant Human CXCL8/IL-8 (Catalog number 208-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (6217) [Unconjugated]

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL8/IL-8 Antibody (MAB208)(200)

Publications using MAB208

• Yang, Q;Guo, H;Li, H;Li, Z;Ni, F;Wen, Z;Liu, K;Kong, H;Wei, W; The CXCL8/MAPK/hnRNP-K axis enables susceptibility to infection by EV-D68, rhinovirus, and influenza virus in vitro Nature communications 2025-02-17 [PMID: 39962077] (Neutralization, Human)
• Joung, J;Heo, Y;Kim, Y;Kim, J;Choi, H;Jeon, T;Jang, Y;Kim, EJ;Lee, SH;Suh, JM;Elledge, SJ;Kim, MS;Kang, C; Cell enlargement modulated by GATA4 and YAP instructs the senescence-associated secretory phenotype Nature communications 2025-02-17 [PMID: 39962062] (Western Blot, Human)
• Cambria, E;Blazeski, A;Ko, EC;Thai, T;Dantes, S;Barbie, DA;Shelton, SE;Kamm, RD; Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis bioRxiv : the preprint server for biology 2025-01-14 [PMID: 39868191] (Neutralization, Human)
• Sun, Y;Maggs, L;Panda, A;Wright, SJ;Cicerchia, AM;Jenney, A;Perricone, MD;Mills, CE;Cattaneo, G;Ventin, M;Chen, F;Rasmussen, MQ;Miranda, A;Revach, OY;Fang, J;Fu, A;Bowling, PJ;Sharova, T;Lawless, A;Sorger, PK;Bardeesy, N;Wang, X;Flaherty, KT;Boland, GM;Mehta, A;Sade-Feldman, M;Ferrone, CR;Jenkins, RW; TBK1 Targeting Is Identified as a Therapeutic Strategy to Enhance CAR T-Cell Efficacy Using Patient-Derived Organotypic Tumor Spheroids Cancer immunology research 2025-01-09 [PMID: 39785827] (Neutralization, Human)
• SenGupta, S;Cohen, E;Serrenho, J;Ott, K;Coulombe, PA;Parent, CA; TGF?1-TNF? regulated secretion of neutrophil chemokines is independent of epithelial-mesenchymal transitions in breast tumor cells bioRxiv : the preprint server for biology 2024-10-12 [PMID: 39416223] (Neutralization, Human)
• Chan, ASL;Zhu, H;Narita, M;Cassidy, LD;Young, ARJ;Bermejo-Rodriguez, C;Janowska, AT;Chen, HC;Gough, S;Oshimori, N;Zender, L;Aitken, SJ;Hoare, M;Narita, M; Titration of RAS alters senescent state and influences tumour initiation Nature 2024-08-07 [PMID: 39112713] (Immunohistochemistry, Western Blot, Mouse)
• Chien, Y;Huang, XY;Yarmishyn, AA;Chien, CS;Liu, YH;Hsiao, YJ;Lin, YY;Lai, WY;Huang, SC;Lee, MS;Chiou, SH;Yang, YP;Chiou, GY; Paracrinal regulation of neutrophil functions by coronaviral infection in iPSC-derived alveolar type II epithelial cells Virus research 2024-07-01 [PMID: 38754785] (Neutralization, Human)
• Kim, HJ;Chang, HK;Lee, YM;Heo, K; Catecholamines Promote Ovarian Cancer Progression through Secretion of CXC-Chemokines International journal of molecular sciences 2023-09-14 [PMID: 37762405] (Neutralization, Human)
• Chen Z, Will R, Kim SN et al. Novel Function of Cancer Stem Cell Marker ALDH1A3 in Glioblastoma: Pro-Angiogenesis through Paracrine PAI-1 and IL-8 Cancers (Basel) 2023-09-04 [PMID: 37686698]
• Chen Z, Will R, Kim SN et al. Novel Function of Cancer Stem Cell Marker ALDH1A3 in Glioblastoma: Pro-Angiogenesis through Paracrine PAI-1 and IL-8 Cancers (Basel) 9/4/2023 [PMID: 37686698]
• Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK; Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation Theranostics 2023-08-21 [PMID: 37649607] (Western Blot, Human)
• Yang, JL;Lin, WL;Tai, SB;Ciou, YS;Chung, CL;Chen, JJ;Liu, PF;Lin, MW;Chen, CL; Suppression of TGF beta -Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38-NF-kB Pathway in Carcinoma Cells International journal of molecular sciences 2023-07-19 [PMID: 37511415] (Western Blot, Human)
• Edmée Eyraud, Elise Maurat, Jean-Marc Sac-Epée, Pauline Henrot, Maeva Zysman, Pauline Esteves et al. Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory response eLife 7/26/2023 [PMID: 37494277]
• Fu, X;Pereira, R;Liu, CC;De Angelis, C;Shea, MJ;Nanda, S;Qin, L;Mitchell, T;Cataldo, ML;Veeraraghavan, J;Sethunath, V;Giuliano, M;Gutierrez, C;Győrffy, B;Trivedi, MV;Cohen, O;Wagle, N;Nardone, A;Jeselsohn, R;Rimawi, MF;Osborne, CK;Schiff, R; High FOXA1 levels induce ER transcriptional reprogramming, a pro-metastatic secretome, and metastasis in endocrine-resistant breast cancer Cell reports 2023-07-18 [PMID: 37467106] (Neutralization, Human)
• Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y; IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer International immunopharmacology 2023-06-16 [PMID: 37331296] (ICC, IHC, Neutralization, Human)
• Smer-Barreto, V;Quintanilla, A;Elliott, RJR;Dawson, JC;Sun, J;Campa, VM;Lorente-Macías, Á;Unciti-Broceta, A;Carragher, NO;Acosta, JC;Oyarzún, DA; Discovery of senolytics using machine learning Nature communications 2023-06-10 [PMID: 37301862] (ICC, Human)
• Huang, J;Wang, X;Wen, J;Zhao, X;Wu, C;Wang, L;Cao, X;Dong, H;Xu, X;Huang, F;Zhu, W;Wang, M; Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPAR?-CPT1A signaling to potentiate lymphatic metastasis Cancer cell international 2023-05-09 [PMID: 37158903] (Neutralization, Human)
• Ming-Na Zhao, Ling-Fei Zhang, Zhen Sun, Li-Hua Qiao, Tao Yang, Yi-Zhe Ren et al. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer Cell Death & Disease 2023-05-01 [PMID: 37127752] (Western Blot, In vivo assay, Neutralization, Human, Mouse)
• Ming-Na Zhao, Ling-Fei Zhang, Zhen Sun, Li-Hua Qiao, Tao Yang, Yi-Zhe Ren et al. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer Cell Death & Disease 5/1/2023 [PMID: 37127752]
• YJ Kahm, IG Kim, RK Kim RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma International Journal of Molecular Sciences, 2023-04-06;24(7):. 2023-04-06 [PMID: 37047826] (Neutralization, Human)
• S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study Scientific Reports, 2022-12-15;12(1):21667. 2022-12-15 [PMID: 36522387] (ELISA Capture, Human)
• Shuhai Chen, Mingyou Chen Schwann cells promote the migration and invasion of colorectal cancer cells via the activated NF-kappa B/IL-8 axis in the tumor microenvironment Frontiers in Oncology 11/17/2022 [PMID: 36465391]
• W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Flow Cytometry, Human)
• S Sun, L Jin, Y Zheng, J Zhu Modeling human HSV infection via a vascularized immune-competent skin-on-chip platform Nature Communications, 2022-09-19;13(1):5481. 2022-09-19 [PMID: 36123328] (Neutralization, Human)
• Thiago Henrique Caldeira Oliveira, Vincent Vanheule, Sofie Vandendriessche, Fariba Poosti, Mauro Martins Teixeira, Paul Proost et al. The GAG-Binding Peptide MIG30 Protects against Liver Ischemia-Reperfusion in Mice International Journal of Molecular Sciences 2022-08-26 [PMID: 36077113] (ELISA Capture, Human)
• Thiago Henrique Caldeira Oliveira, Vincent Vanheule, Sofie Vandendriessche, Fariba Poosti, Mauro Martins Teixeira, Paul Proost et al. The GAG-Binding Peptide MIG30 Protects against Liver Ischemia-Reperfusion in Mice International Journal of Molecular Sciences 8/26/2022 [PMID: 36077113]
• MF Santolla, M Talia, F Cirillo, D Scordamagl, S De Rosis, A Spinelli, AM Miglietta, B Nardo, G Filippelli, EM De Frances, A Belfiore, R Lappano, M Maggiolini The AGEs/RAGE Transduction Signaling Prompts IL-8/CXCR1/2-Mediated Interaction between Cancer-Associated Fibroblasts (CAFs) and Breast Cancer Cells Cells, 2022-08-04;11(15):. 2022-08-04 [PMID: 35954247] (Bioassay, Human)
• R. C. Cornelison, J. X. Yuan, K. M. Tate, A. Petrosky, G. F. Beeghly, M. Bloomfield et al. A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment npj Precision Oncology 7/29/2022 [PMID: 35906273]
• R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance Oncogene, 2022-07-19;40(3):111097. 2022-07-19 [PMID: 35858551] (Western Blot, Human)
• Haoran Zhu, Keefe T Chan, Xinran Huang, Carmelo Cerra, Shaun Blake, Anna S Trigos et al. Cystathionine-beta -synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activation eLife 6/27/2022 [PMID: 35758651]
• Hoai-Nga Thi Nguyen, Marie Kawahara, Cat-Khanh Vuong, Mizuho Fukushige, Toshiharu Yamashita, Osamu Ohneda SARS-CoV-2 M Protein Facilitates Malignant Transformation of Breast Cancer Cells Frontiers in Oncology 6/7/2022 [PMID: 35747796]
• Valerio Ciccone, Erika Terzuoli, Emma Ristori, Arianna Filippelli, Marina Ziche, Lucia Morbidelli et al. ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL‑8/Notch signaling cascade International Journal of Molecular Medicine 2022-06-02 [PMID: 35656893] (Neutralization, Human)
• Valerio Ciccone, Erika Terzuoli, Emma Ristori, Arianna Filippelli, Marina Ziche, Lucia Morbidelli et al. ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL‑8/Notch signaling cascade International Journal of Molecular Medicine 6/2/2022 [PMID: 35656893]
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Review for CXCL8/IL-8 Antibody (MAB208) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 08/30/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human lymph node (melanoma)
• Species: Human
• Lot: ASJ3517041

Comments

• Comments: Human FFPE metastatic melanoma lymph node sections were stained with anti-human IL-8 monoclonal antibody (MAB208) at 5 µg/ml followed by secondary goat anti-mouse biotinylated antibody and Streptavidin AP conjugate. Detection via an Alkaline Phosphatase/Red staining System.
  Blocking: 5 % goat Serum in TBS-T
  Antigen Retrieval: Citrat buffer
  Primary antibody incubation: over night at 4 °C

Bioinformatics

• Gene Symbol: IL8
• Uniprot:
  • P10145()