Human CXCL7/NAP-2 ELISA Kit (Colorimetric)

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ELISA: Human CXCL7/NAP-2 ELISA Kit (Colorimetric) [NBP2-59965]
Human CXCL7/NAP-2 ELISA Kit (Colorimetric) [NBP2-59965] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
78 pg/mL - 5000 pg/mL
Sensitivity
19.5 pg/mL

Order Details

Human CXCL7/NAP-2 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human NAP-2. No significant cross-reactivity or interference between human NAP-2 and analogues was observed.
Standard Curve Range
78 pg/mL - 5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PPBP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human CXCL7/NAP-2 ELISA Kit (Colorimetric)

  • Beta-TG
  • connective tissue-activating peptide III
  • CTAP III
  • CTAP3CTAP-III
  • CTAPIII
  • CXC chemokine ligand 7
  • C-X-C motif chemokine 7
  • CXCL7
  • CXCL7b-TG1
  • LA-PF4
  • LDGFTC1
  • Leukocyte-derived growth factor
  • low-affinity platelet factor IV
  • Macrophage-derived growth factor
  • MDGFTC2
  • NAP2
  • NAP-2
  • NAP-2-L1
  • neutrophil-activating peptide 2
  • neutrophil-activating peptide-2
  • PBPTHBGB
  • platelet basic protein
  • PPBP
  • pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
  • SCAR10
  • SCYB7beta-thromboglobulin
  • small inducible cytokine B7
  • small inducible cytokine subfamily B, member 7
  • Small-inducible cytokine B7
  • TGB
  • TGB1B-TG1
  • THBGB1
  • thrombocidin 1
  • thrombocidin 2
  • thromboglobulin, beta-1

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PPBP