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333-GC) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP-2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP-2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 µg/mL." class="big_lightbox" target="_blank">
333-GC) was serially diluted and captured by Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL6/GCP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF333) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
333-GC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL6/ GCP-2 (80 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CXCL6/GCP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF333). The ND50 is typically 0.8-4.0 µg/mL." class="big_lightbox" target="_blank">
333-GC) was serially diluted and captured by Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL6/GCP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF333) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">