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Human CXCL4/PF4 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1761
    • Availability
      Product Discontinued

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Human CXCL4/PF4 ELISA Kit (Colorimetric) Summary

Sensitivity
20 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PF4

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
PF4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PF4.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent B (Item E): 5x concentrated buffer.
  2. Assay Diluent C (Item L): Diluent buffer.
  3. Detection Antibody PF-4 (Item F): biotinylated anti-human PF-4 (each vial isenough to assay half microplate).
  4. HRP-Streptavidin concentrates (Item G): 500x concentrated HRP-conjugatedStreptavidin.
  5. PF-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-humanPF-4.
  6. Standards (Item C): Human PF-4. 1 vial is enough to run each standard induplicate.
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): of 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human CXCL4/PF4 ELISA Kit (Colorimetric)

  • chemokine (C-X-C motif) ligand 4
  • C-X-C motif chemokine 4
  • CXCL4
  • CXCL4iroplact
  • Iroplact
  • MGC138298
  • Oncostatin-A
  • PF4
  • PF-4
  • platelet factor 4
  • SCYB4oncostatin-A

Background

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for CXCL4/PF4 ELISA Kit (KA1761). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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  CXCL4/PF4 ELISA Kit [Biotin]
  PDF Datasheet
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Bioinformatics

Gene Symbol PF4