Human CXCL4/PF4 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1761
    • Availability
      Product Discontinued

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Human CXCL4/PF4 ELISA Kit (Colorimetric) Summary

Sensitivity
20 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PF4

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
PF4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PF4.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent B (Item E): 5x concentrated buffer.
  2. Assay Diluent C (Item L): Diluent buffer.
  3. Detection Antibody PF-4 (Item F): biotinylated anti-human PF-4 (each vial isenough to assay half microplate).
  4. HRP-Streptavidin concentrates (Item G): 500x concentrated HRP-conjugatedStreptavidin.
  5. PF-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-humanPF-4.
  6. Standards (Item C): Human PF-4. 1 vial is enough to run each standard induplicate.
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): of 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human CXCL4/PF4 ELISA Kit (Colorimetric)

  • chemokine (C-X-C motif) ligand 4
  • C-X-C motif chemokine 4
  • CXCL4
  • CXCL4iroplact
  • Iroplact
  • MGC138298
  • Oncostatin-A
  • PF4
  • PF-4
  • platelet factor 4
  • SCYB4oncostatin-A

Background

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for CXCL4/PF4 ELISA Kit (KA1761). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol PF4