CXCL4/PF4 Antibody

Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody [NBP1-50290] - Analysis of anti-PF4 antibody with human placenta at concentration 5 ug/ml.

Product Discontinued

CXCL4/PF4 Antibody Summary

• Immunogen: CXCL4/PF4 purified from human platelet releasate.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PF4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23754740).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.4), 0.15M NaCl, and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCL4/PF4 Antibody

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL4/PF4 Antibody (NBP1-50290)(1)

Publication using NBP1-50290

• Zhang S, Mercado-Uribe I, Liu J. Tumor stroma and differentiated cancer cells can be originated directly from polyploid giant cancer cells induced by paclitaxel. Int J Cancer 2013-06-10 [PMID: 23754740] (ICC/IF, Human)

FAQs for CXCL4/PF4 Antibody (NBP1-50290). (Showing 1 - 1 of 1 FAQ).

1. I would like to know if you have any recommendation/suggestion regarding antigen retrieval method. Can you share the IHC protocol for #NBP1-50290 with me?
   • For this product we recommend heat induced antigen retrieval with citrate buffer pH 6.0, see here.

Bioinformatics

• Gene Symbol: PF4
• Entrez:
  • 5196(Human)
• Uniprot:
  • P02776()