CXCL2/GRO beta/MIP-2/CINC-3 Antibody [Biotin]

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CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody ...read more
CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed frozen sections of mouse spleen using Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ELISA(Det), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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CXCL2/GRO beta/MIP-2/CINC-3 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse CXCL2/MIP-2 (R&D Systems, Catalog # 452-M2)
Ala28-Asn100
Accession # P10889
Specificity
Detects mouse CXCL2/MIP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) GRO beta, rhGRO gamma, recombinant mouse (rm) C10, rmMIP-1 alpha, rmMIP-1 beta, rhRANTES, and rmKC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL2
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody (Catalog # MAB452)
ELISA Detection: Mouse CXCL2/GRO beta/MIP-2/CINC-3 Biotinylated Antibody (Catalog # BAF452)
Standard: Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2)
Readout System
Reviewed Applications
Read 1 Review rated 4
using
BAF452 in the following applications:

Publications
Read Publications using
BAF452 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL2/GRO beta/MIP-2/CINC-3 Antibody [Biotin]

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2

Background

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (BAF452)(19)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ELISA Detection, ELISA Development, ELISA Development (Detection).


Filter By Application
ELISA Detection
(1)
ELISA Development
(12)
ELISA Development (Detection)
(6)
All Applications
Filter By Species
Mouse
(19)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using BAF452 Applications Species
Z Pang, R Raudonis, C McCormick, Z Cheng Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection Infect. Immun., 2019;0(0):. 2019 [PMID: 31611276] (ELISA Detection, Mouse) ELISA Detection Mouse
M Muccioli, H Nandigam, T Loftus, M Singh, A Venkatesh, J Wright, M Pate, K McCall, F Benencia Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues Oncotarget, 2018;9(94):36666-36683. 2018 [PMID: 30613350] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells Immunobiology, 2017;0(0):. 2017 [PMID: 29331323] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 29018150] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017;42(5):1961-1972. 2017 [PMID: 28793286] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Immunity, 0;43(5):945-58. 0 [PMID: 26588780] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
Junkins R, MacNeil A, Wu Z, McCormick C, Lin T Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol, 2013;190(10):5178-86. 2013 [PMID: 23589609] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Am. J. Pathol., 2011;179(6):2730-9. 2011 [PMID: 21967814] (ELISA Development, Mouse) ELISA Development Mouse
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009;0(0):. 2009 [PMID: 19592492] (ELISA Development, Mouse) ELISA Development Mouse
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009;11(4):524-30. 2009 [PMID: 19286469] (ELISA Development, Mouse) ELISA Development Mouse
Show All 19 Publications.

Review for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (BAF452) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using BAF452:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IHC-Fr Mouse 01/14/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID: 22160719
SpeciesMouse
Special ApplicationsIndependent review from antibodyresource.comSee PMID: 22160719 for more details.

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Biotin BAF452

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Recent Reviews

4
5
0
4
1
3
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2
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1
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Anonymous
01/14/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CXCL2
Uniprot