CXCL2/GRO beta/MIP-2/CINC-3 Antibody [Biotin]

CXCL2/GRO beta /MIP-2/CINC-3 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CXCL2/GRO beta /MIP-2/CINC-3 was detected in immersion fixed frozen sections of mouse spleen using Mouse CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL2/GRO beta/MIP-2/CINC-3 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL2/MIP-2 (R&D Systems, Catalog # 452-M2)
  Ala28-Asn100
  Accession # P10889
• Specificity: Detects mouse CXCL2/MIP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) GRO beta , rhGRO gamma , recombinant mouse (rm) C10, rmMIP-1 alpha , rmMIP-1 beta , rhRANTES, and rmKC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody (Catalog # MAB452)
  ELISA Detection: Mouse CXCL2/GRO beta/MIP-2/CINC-3 Biotinylated Antibody (Catalog # BAF452)
  Standard: Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL2/GRO beta/MIP-2/CINC-3 Antibody [Biotin]

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROB
• Gro-beta
• Growth-regulated protein beta
• Macrophage inflammatory protein 2-alpha
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma . It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (BAF452)(19)

Publications using BAF452

• Z Pang, R Raudonis, C McCormick, Z Cheng Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection Infect. Immun., 2019-12-17;0(0):. 2019-12-17 [PMID: 31611276] (ELISA Detection, Mouse)
• M Muccioli, H Nandigam, T Loftus, M Singh, A Venkatesh, J Wright, M Pate, K McCall, F Benencia Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues Oncotarget, 2018-11-30;9(94):36666-36683. 2018-11-30 [PMID: 30613350] (ELISA Development (Detection), Mouse)
• A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells Immunobiology, 2017-11-22;0(0):. 2017-11-22 [PMID: 29331323] (ELISA Development (Detection), Mouse)
• Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection J. Leukoc. Biol., 2017-10-10;0(0):. 2017-10-10 [PMID: 29018150] (ELISA Development (Detection), Mouse)
• R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Detection), Mouse)
• Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Immunity, 2015-11-17;43(5):945-58. 2015-11-17 [PMID: 26588780] (ELISA Development (Detection), Mouse)
• Junkins R, MacNeil A, Wu Z, McCormick C, Lin T Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol, 2013-04-15;190(10):5178-86. 2013-04-15 [PMID: 23589609] (ELISA Development (Detection), Mouse)
• Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Am. J. Pathol., 2011-10-01;179(6):2730-9. 2011-10-01 [PMID: 21967814] (ELISA Development, Mouse)
• Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009-07-10;0(0):. 2009-07-10 [PMID: 19592492] (ELISA Development, Mouse)
• Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009-03-13;11(4):524-30. 2009-03-13 [PMID: 19286469] (ELISA Development, Mouse)
• Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA Development, Mouse)
• Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. J. Immunol., 2006-12-15;177(12):8650-7. 2006-12-15 [PMID: 17142765] (ELISA Development, Mouse)
• Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect. Immun., 2006-09-18;74(12):6730-8. 2006-09-18 [PMID: 16982824] (ELISA Development, Mouse)
• Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Eur. J. Immunol., 2006-08-01;36(8):2025-34. 2006-08-01 [PMID: 16856209] (ELISA Development, Mouse)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA Development, Mouse)
• Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. J. Immunol., 2006-07-01;177(1):612-20. 2006-07-01 [PMID: 16785559] (ELISA Development, Mouse)
• Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation. J. Immunol., 2005-08-15;175(4):2589-97. 2005-08-15 [PMID: 16081833] (ELISA Development, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse)
• Jeyaseelan S, Chu HW, Young SK, Worthen GS Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Infect. Immun., 2004-12-01;72(12):7247-56. 2004-12-01 [PMID: 15557650] (ELISA Development, Mouse)

Review for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (BAF452) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 01/14/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID: 22160719
• Species: Mouse

Bioinformatics

• Gene Symbol: CXCL2
• Uniprot:
  • P10889()