CXCL14/BRAK Antibody (131120)

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 131120
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL14/BRAK Antibody (131120) Summary

• Immunogen: E. coli-derived recombinant human CXCL14/BRAK
  Leu23-Tyr99
  Accession # O95715
• Specificity: Detects human CXCL14/BRAK in ELISAs and Western blots. In direct ELISAs, shows approximately 80% cross-reactivity with recombinant mouse (rm) CXCL14/BRAK and no cross-reactivity with recombinant human (rh)GRO alpha , rhGRO beta , rhGRO gamma ,rhMIP-1 alpha , rmMIP-1 alpha , rmMIP-1 beta , rhMIP-1δ, rmMIP-1 gamma , rmMIP-2, rhMIP-3 alpha , rmMIP-3 alpha , rrMIP-3 alpha , rhMIP-3 beta , or rmMIP-3 beta .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CXCL14/BRAK Biotinylated Antibody (Catalog number BAM8661)
  Standard: Recombinant Human CXCL14/BRAK (Catalog number 866-CX)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL14/BRAK Antibody (131120)

• BMAC
• Bolekine
• BRAK
• BRAKKEC
• chemokine (C-X-C motif) ligand 14
• Chemokine BRAK
• CXCL14
• CXC-X3
• member 14 (BRAK)
• MIP-2 gamma
• MIP2G
• MIP-2G
• NJACKec
• SCYB14MGC10687
• Small-inducible cytokine B14

Background

CXCL14/BRAK (breast and kidney-expressed chemokine), also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Human CXCL14 shares approximately 30% aa sequence identity with MIP-2 alpha (GRO beta ) as well as MIP-2 beta  (GRO gamma ). The gene for CXCL14 has been mapped human chromosome 5q31. Unlike the MIP-2 chemokines, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E₂ or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important for regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
2. Cao, X. et al. (2000) J. Immunol. 165:2588.
3. Kurth, I. et al. (2001) J. Exp., Med. 194:855.
4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL14/BRAK Antibody (MAB866)(5)

Publications using MAB866

• Xiaotong Mao, Shenghan Mao, Lei Wang, Hui Jiang, Shunjiang Deng, Yuanchen Wang et al. Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis Genes (Basel) 2022-06-05 [PMID: 35741777] (Immunohistochemistry, Mouse)
• Sturmlechner I, Zhang C, Sine CC Et al. p21 produces a bioactive secretome that places stressed cells under immunosurveillance Science (New York, N.Y.) 10/29/2021 [PMID: 34709885]
• B Fazi, C Proserpio, S Galardi, F Annesi, M Cola, A Mangiola, A Michienzi, SA Ciafrè The Expression of the Chemokine CXCL14 Correlates with Several Aggressive Aspects of Glioblastoma and Promotes Key Properties of Glioblastoma Cells Int J Mol Sci, 2019-05-21;20(10):. 2019-05-21 [PMID: 31117166] (Western Blot, Human)
• Takiguchi S, Korenaga N, Inoue K, Sugi E, Kataoka Y, Matsusue K, Futagami K, Li Y, Kukita T, Teramoto N, Iguchi H Involvement of CXCL14 in osteolytic bone metastasis from lung cancer. Int J Oncol, 2014-02-10;44(4):1316-24. 2014-02-10 [PMID: 24534874] (IHC-P, Human, Mouse)
• Shurin GV, Ferris RL, Ferris R, Tourkova IL, Perez L, Lokshin A, Balkir L, Collins B, Chatta GS, Shurin MR Loss of new chemokine CXCL14 in tumor tissue is associated with low infiltration by dendritic cells (DC), while restoration of human CXCL14 expression in tumor cells causes attraction of DC both in vitro and in vivo. J. Immunol., 2005-05-01;174(9):5490-8. 2005-05-01 [PMID: 15843547] (ICC, IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: CXCL14
• Uniprot:
  • O95715()