CXCL11/I-TAC Antibody [Unconjugated]

Recombinant HumanCXCL11/I‑TAC (Catalog #672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCXCL11/I‑TAC (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL11/I-TAC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF260). The ND50 is typically 0.5-1.5 µg/mL.

CXCR7-mediated Rb degradation requires ligand.pLEC cultures were infected with Trans only or Trans+CXCR7. At 6 hours post-infection, media was replaced with media only or media containing (A) SDF-1/CXCL12 neutralizing antibody at 1.5, or 3.0 µg/ml or (B) ITAC/CXCL11 neutralizing antibody at 0.5 or 1.0 µg/ml. At 20 hours post infection, cells were lysed and analyzed by western blot for total Rb levels. Densitometry analysis was performed and fold change values were calculated from GAPDH-normalized optical density ratios as described for Figure 3. Bar graphs (bottom) represent the average fold change at each condition for two independent experiments. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0069828), licensed under a CC-BY license. Not internally tested by R&D Systems.

CXCR7-mediated Rb degradation requires ligand.pLEC cultures were infected with Trans only or Trans+CXCR7. At 6 hours post-infection, media was replaced with media only or media containing (A) SDF-1/CXCL12 neutralizing antibody at 1.5, or 3.0 µg/ml or (B) ITAC/CXCL11 neutralizing antibody at 0.5 or 1.0 µg/ml. At 20 hours post infection, cells were lysed and analyzed by western blot for total Rb levels. Densitometry analysis was performed and fold change values were calculated from GAPDH-normalized optical density ratios as described for Figure 3. Bar graphs (bottom) represent the average fold change at each condition for two independent experiments. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0069828), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL11/I-TAC Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL11/I-TAC
  Phe22-Phe94
  Accession # O14625
• Specificity: Detects human CXCL11/I-TAC in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse
  I-TAC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL11
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-1.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL11/I-TAC Antibody [Unconjugated]

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

1. Cole, K. et al. (1998) J. Exp. Med. 187:2009.
2. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
3. Lou, Y. et al. (1998) J. Neurovirol. 4:575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL11/I-TAC Antibody (AF260)(3)

Publications using AF260

• H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices IBRO Rep, 2018-11-28;6(0):1-17. 2018-11-28 [PMID: 30582064] (IHC-Fr, Mouse)
• Jennifer E. Totonchy, Jessica M. Osborn, Sara Botto, Lisa Clepper, Ashlee V. Moses Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein PLoS ONE 7/23/2013 [PMID: 23894550]
• Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. J. Immunol., 2009-03-15;182(6):3919-27. 2009-03-15 [PMID: 19265171] (IHC-Fr, Human)

Review for CXCL11/I-TAC Antibody (AF260) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19552626
• Species: Human

Bioinformatics

• Gene Symbol: CXCL11
• Uniprot:
  • O14625()