CXCL1/2/3/GRO Antibody (186413)

Recombinant Cotton Rat CXCL1/2/3/GRO (Catalog # 1114-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CXCL1/2/3/GRO (5 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB1114). The ND50 is typically 0.08-0.4 µg/mL.

• Reactivity: Cr
• Applications: WB, B/N
• Clone: 186413
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL1/2/3/GRO Antibody (186413) Summary

• Immunogen: E. coli-derived recombinant cotton rat GRO
  Ala28-Lys100
  Accession # AAL16934
• Specificity: Detects cotton rat CXCL1/2/3/GRO in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 5-20% cross-reactivity with recombinant rat CXCL1 and recombinant human CXCL2 and no cross-reactivity with recombinant cotton rat CXCL10, recombinant human CXCL1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha , rh12/SDF-1 beta , 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11, 12/SDF-1 alpha , 13, 15, 16, 17, recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha , rr3/CINC-2 beta , or recombinant porcine CXCL8.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.08-0.4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/2/3/GRO Antibody (186413)

• CXCL1/2/3
• FSP
• GRO1
• GROa
• MGSA
• MGSA-a
• NAP-3
• SCYB1

Background

Cotton rat growth-regulated protein (GRO) is a member of the ELR⁺ CXC subfamily of chemokines (1). The cotton rat GRO cDNA encodes a 100 amino acid (aa) precursor protein with a 27 aa putative signal peptide and a 73 aa mature protein (2). Among the three human GRO proteins, mature cotton rat GRO is most closely related to CXCL1/GRO alpha , sharing approximately 77% aa sequence identity (3). It also shares 75% and 70% aa sequence identity with mature human CXCL2/GRO beta and CXCL3/GRO gamma , respectively (4). Compared with rat CXC chemokines, mature cotton rat shows 92%, 76%, and 76% aa sequence homology with rat KC/GRO/CINC-1, CINC-2, and CINC-3, respectively (5). Mature cotton rat CXCL1 also shares 74% sequence homology with mature cotton rat MIP-2 (6). Similarly to GRO proteins from other species, cotton rat CXCL1 is expected to be a potent neutrophil chemoattractant and activator. Cotton rat CXCL1 has been shown to bind and activate both mouse chemokine receptors CXCR1 and CXCR2 (7). Similarly to human CXCL1, the cotton rat CXCL1 concentration required to activate CXCR1 is at least an order of magnitude higher than that required to activate CXCR2 (7, 8). Based on studies on rodents, cotton rat CXCL1 is predicted to be produced by a wide variety of cell types including macrophages, endothelial cells, fibroblasts, astrocytes, neutrophils, keratinocytes, and intestinal epithelium (1, 9).

1. Rollins, B.J. (1997) Blood 90:909.
2. Blanco, J.C. et al. (2001) GenBank Accession #: AAL16934.
3. Richmond, A. et al. (1988) EMBO J. 7:2025.
4. Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
5. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
6. Blanco, J.C. et al. (2001) GenBank Accession #: AAL26705.
7. R&D Systems, unpublished observations.
8. Schumacher, C. et al. (1992) Proc. Natl. Acad. Sci. USA 89:10542.
9. Zlotnik, A.O. (2000) Immunity 12:121.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAL16934()