CXCL1/2/3/GRO Antibody (186413) [Alexa Fluor® 532]

• Reactivity: Cr
• Applications: WB, B/N
• Clone: 186413
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

CXCL1/2/3/GRO Antibody (186413) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant cotton rat GRO
  Ala28-Lys100
  Accession # AAL16934
• Specificity: Detects cotton rat CXCL1/2/3/GRO in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 5-20% cross-reactivity with recombinant rat CXCL1 and recombinant human CXCL2 and no cross-reactivity with recombinant cotton rat CXCL10, recombinan
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/2/3/GRO Antibody (186413) [Alexa Fluor® 532]

• CXCL1/2/3
• FSP
• GRO1
• GROa
• MGSA
• MGSA-a
• NAP-3
• SCYB1

Background

Cotton rat growth-regulated protein (GRO) is a member of the ELR⁺ CXC subfamily of chemokines (1). The cotton rat GRO cDNA encodes a 100 amino acid (aa) precursor protein with a 27 aa putative signal peptide and a 73 aa mature protein (2). Among the three human GRO proteins, mature cotton rat GRO is most closely related to CXCL1/GRO alpha , sharing approximately 77% aa sequence identity (3). It also shares 75% and 70% aa sequence identity with mature human CXCL2/GRO beta and CXCL3/GRO gamma , respectively (4). Compared with rat CXC chemokines, mature cotton rat shows 92%, 76%, and 76% aa sequence homology with rat KC/GRO/CINC-1, CINC-2, and CINC-3, respectively (5). Mature cotton rat CXCL1 also shares 74% sequence homology with mature cotton rat MIP-2 (6). Similarly to GRO proteins from other species, cotton rat CXCL1 is expected to be a potent neutrophil chemoattractant and activator. Cotton rat CXCL1 has been shown to bind and activate both mouse chemokine receptors CXCR1 and CXCR2 (7). Similarly to human CXCL1, the cotton rat CXCL1 concentration required to activate CXCR1 is at least an order of magnitude higher than that required to activate CXCR2 (7, 8). Based on studies on rodents, cotton rat CXCL1 is predicted to be produced by a wide variety of cell types including macrophages, endothelial cells, fibroblasts, astrocytes, neutrophils, keratinocytes, and intestinal epithelium (1, 9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAL16934()