CX3CR1 Antibody (528728) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human CX3CR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5204P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Human PBMC were stained with (A) Mouse Anti-Human CX3CR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5204P) or (B) Mouse IgG1 isotype control antibody (Catalog # IC002P) and Mouse anti-Human CD14 APC-conjugated antibody (Catalog # FAB3832A). Staining was done at 37oC. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 528728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CX3CR1 Antibody (528728) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human CX3CR1
  Met1-Thr31, Leu91-Lys103, Thr168-Thr195, Lys257-Leu273
  Accession # NP_001328
• Specificity: Detects human CX3CR1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CX3CR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CR1 Antibody (528728) [Phycoerythrin]

• Beta chemokine receptor-like 1
• CCRL1
• chemokine (C-C) receptor-like 1
• chemokine (C-X3-C motif) receptor 1
• chemokine (C-X3-C) receptor 1
• CMKbRL1
• CMK-BRL1
• CMK-BRL-1
• CMKDR1
• CX3C chemokine receptor 1
• CX3CR1
• Fractalkine receptor
• G protein-coupled receptor 13
• GPR13G-protein coupled receptor 13
• GPRV28
• V28
• V28C-X3-C CKR-1

Background

CX3CR1 is a 40 kDa seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia, and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion co-receptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CR1 Antibody (FAB5204P)(6)

Publications using FAB5204P

• Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience, 2021-11-14;24(12):103433. 2021-11-14 [PMID: 34917892] (Flow Cytometry, Human)
• S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial Biomed Res Int, 2020-07-27;2020(0):8748934. 2020-07-27 [PMID: 32802883] (Flow Cytometry, Human)
• Karoline Walscheid Phenotypic changes of peripheral blood mononuclear cells upon corticosteroid treatment in idiopathic intermediate uveitis Clin. Immunol, 2016-10-28;0(0):. 2016-10-28 [PMID: 27989897] (Flow Cytometry, Human)
• Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD. PLoS ONE, 2014-12-15;9(12):e112473. 2014-12-15 [PMID: 25503251] (Flow Cytometry, Human)
• Carrega , Paolo, Bonaccorsi , Irene, Di Carlo , Emma, Morandi , Barbara, Paul , Petra, Rizzello , Valeria, Cipollone , Giuseppe, Navarra , Giuseppe, Mingari , Maria Cr, Moretta , Lorenzo, Ferlazzo , Guido CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol, 2014-03-19;192(8):3805-15. 2014-03-19 [PMID: 24646734] (Flow Cytometry, Human)
• Di Pardo A, Alberti S, Maglione V, Amico E, Cortes E, Elifani F, Battaglia G, Busceti C, Nicoletti F, Vonsattel J, Squitieri F Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease. Mol Brain, 2013-12-13;6(0):55. 2013-12-13 [PMID: 24330808] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CX3CR1
• Uniprot:
  • NP_001328()