Recombinant Rat CX3CL1/Fractalkine Protein

Images

 
SDS-Page: Rat CX3CL1/Fractalkine Protein [NBP2-35198]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rat CX3CL1/Fractalkine Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain consisting a chemokine domain of Human Fracktalkine and corresponding to 76 residues of CX3CL1.

Source: E. coli

Amino Acid Sequence: QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
CX3CL1 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-10 ng/ml.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
CX3CL1
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
8.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 2x PBS, pH 7.4.
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Rat CX3CL1/Fractalkine Protein

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Fractalkine/CX3CL1
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Rat CX3CL1 contains 76 amino acids and it shares approximately 86 % and 83 % amino acid sequence homology with the murine and human protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-76949
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P
AF477
Species: Hu, Mu
Applications: WB, IHC, Block
NBP2-29460
Species: Hu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PAGE
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
AF482
Species: Mu
Applications: WB, IHC
NBP2-35198
Species: Rt
Applications: Func, PAGE

Publications for CX3CL1/Fractalkine Protein (NBP2-35198) (0)

There are no publications for CX3CL1/Fractalkine Protein (NBP2-35198).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CX3CL1/Fractalkine Protein (NBP2-35198) (0)

There are no reviews for CX3CL1/Fractalkine Protein (NBP2-35198). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CX3CL1/Fractalkine Protein (NBP2-35198) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CX3CL1/Fractalkine Products

Bioinformatics Tool for CX3CL1/Fractalkine Protein (NBP2-35198)

Discover related pathways, diseases and genes to CX3CL1/Fractalkine Protein (NBP2-35198). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CX3CL1/Fractalkine Protein (NBP2-35198)

Discover more about diseases related to CX3CL1/Fractalkine Protein (NBP2-35198).
 

Pathways for CX3CL1/Fractalkine Protein (NBP2-35198)

View related products by pathway.

PTMs for CX3CL1/Fractalkine Protein (NBP2-35198)

Learn more about PTMs related to CX3CL1/Fractalkine Protein (NBP2-35198).
 

Research Areas for CX3CL1/Fractalkine Protein (NBP2-35198)

Find related products by research area.

Blogs on CX3CL1/Fractalkine

There are no specific blogs for CX3CL1/Fractalkine, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat CX3CL1/Fractalkine Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol CX3CL1
Entrez
Uniprot