CUEDC2 Antibody

Product Discontinued

CUEDC2 Antibody Summary

• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2. The immunogen is located within the last 50 amino acids of CUEDC2.
• Specificity: CUEDC2 antibody will not cross-react with CUEDC1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUEDC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CUEDC2 Antibody

• bA18I14.5
• C10orf66
• chromosome 10 open reading frame 66
• CUE domain containing 2
• CUE domain-containing protein 2
• MGC2491

Background

The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-kappaB by decreasing the activation of IKK. CUEDC2 antibody will not cross-react with CUEDC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CUEDC2
• Uniprot:
  • NP_076945(Human)