CTRP7 Antibody (K14-S)

Western Blot: CTRP7 Antibody (K14-S) [NBP1-30132] - CTRP7 in Rat (I.) and Mouse (II.) brain crude lysates (200 ug of total protein loaded).

Product Discontinued

CTRP7 Antibody (K14-S) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Peptide derived from internal sequence of human CTRP7. Antibody recognizes the epitope located between Thr220 - Ala231.
• Specificity: This antibody is specific to the carboxy terminus of CTRP7
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: C1QTNF7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:50000
  • Western Blot 1:5000
• Application Notes: For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Tween-20. Blocking buffer: 1xTBS; 0.2% Tween-20; 8% nonfat dry milk.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for CTRP7 Antibody (K14-S)

• C1q and tumor necrosis factor related protein 7
• C1qTNF7
• complement-c1q tumor necrosis factor-related protein 7
• CTRP7
• CTRP7complement C1q tumor necrosis factor-related protein 7
• ZACRP7

Background

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-a-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Like the other members of the adiponectin and CTRP protein family, the mature CTRP7 is secreted and can be found in the organism's circulatory system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C1QTNF7
• Entrez:
  • 114905(Human)
• Uniprot:
  • Q9BXJ2(Human)