Ctip1 Antibody (BCL11A/123) - BSA Free

Western Blot: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of various lysates of transfectants expressing different BCL11A isoforms and the highly homologous BCL11B to show specificity for BCL11AXL.

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of Ctip1 expression in tonsil BCL11AXL is present in B cells (brown) in germinal centres and mantle zones. Double labelling studies show the strongly labelled cells in the interfollicular areas to be CD123-positive pDCs (arrowhead).

Immunohistochemistry: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - BCL11AXL (BCL123a) expression in tonsil BCL11AXL is present in B cells (brown) in germinal centres and mantle zones.

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - BCL11AXL is absent in plasma cells BCL11A+ cells (blue) /CD3+ T cells (red) / p63-positive plasma cells (green)

Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Nuclear staining of COS/ BCL11AXL transfectants but not BCL BCL11AL transfectants using antibody BCL123a.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: BCL11A/123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ctip1 Antibody (BCL11A/123) - BSA Free Summary

• Immunogen: C-terminus of extra long isoform of Ctip1, BCL11AXL (aa 637-835)
• Specificity: Specifically recognises the extra long form of the protein and not the long or short forms of Ctip1. Not cross reactive with the long form of the BCL11B protein that shares significant sequence identity with the immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL11A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: IHC: Frozen use neat supernatant. WB: use neat supernatant. Positive control(s): Tonsil (in B cells and plasmcytoid dendritic cells), Neurones and nuclei of some epithelium.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ctip1 Antibody (BCL11A/123) - BSA Free

• B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
• B-cell CLL/lymphoma 11A (zinc finger protein)
• B-cell CLL/lymphoma 11A
• B-cell lymphoma/leukemia 11A
• BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• BCL-11A
• BCL11A-S
• BCL11A-XL
• C2H2-type zinc finger protein
• COUP-TF-interacting protein 1
• CTIP1ecotropic viral integration site 9
• ecotropic viral integration site 9 homolog
• Ecotropic viral integration site 9 protein homolog
• EVI-9
• EVI9FLJ34997
• HBFQTL5
• KIAA1809BCL11A-L
• Zinc finger protein 856
• ZNF856FLJ10173

Background

The Ctip1 gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, throug

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL11A