Ctip1 Antibody (BCL11A/123) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: BCL11A/123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Ctip1 Antibody (BCL11A/123) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: C-terminus of extra long isoform of Ctip1, BCL11AXL (aa 637-835)
• Specificity: Specifically recognises the extra long form of the protein and not the long or short forms of Ctip1. Not cross reactive with the long form of the BCL11B protein that shares significant sequence identity with the immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL11A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Ctip1 Antibody (BCL11A/123) [CoraFluor™ 1]

• B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
• B-cell CLL/lymphoma 11A (zinc finger protein)
• B-cell CLL/lymphoma 11A
• B-cell lymphoma/leukemia 11A
• BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• BCL-11A
• BCL11A-S
• BCL11A-XL
• C2H2-type zinc finger protein
• COUP-TF-interacting protein 1
• CTIP1ecotropic viral integration site 9
• ecotropic viral integration site 9 homolog
• Ecotropic viral integration site 9 protein homolog
• EVI-9
• EVI9FLJ34997
• HBFQTL5
• KIAA1809BCL11A-L
• Zinc finger protein 856
• ZNF856FLJ10173

Background

The Ctip1 gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, throug

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL11A