CtIP Antibody (19E8)

Images

 
Immunocytochemistry/ Immunofluorescence: CtIP Antibody (19E8) [NBP1-41205] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CtIP protein stained by CtIP antibody [19E8] diluted at 1:100. Red: ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IP, PLA
Clone
19E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CtIP Antibody (19E8) Summary

Immunogen
Human CtIP
Specificity
This antibody will recognize human CtIP.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RBBP8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunoprecipitation
  • Proximity Ligation Assay
Application Notes
ChIP and IP usages reported in scientific literature.
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-41205 in the following applications:

  • 3 publications
  • IP
    3 publications
  • WB
    3 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for CtIP Antibody (19E8)

  • CtBP-interacting protein
  • CtIP
  • DNA endonuclease RBBP8
  • EC 3.1
  • RBBP-8
  • retinoblastoma binding protein 8
  • Retinoblastoma-binding protein 8
  • Retinoblastoma-interacting protein and myosin-like
  • RIMSAE2
  • Sporulation in the absence of SPO11 protein 2 homolog

Background

CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disruppted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-598
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NB100-143
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, IB, ICC/IF, IHC, IHC-P, IP, ISH, PAGE, KO
NB100-147
Species: Hu, Mu, Rt, Mk
Applications: WB, ICC/IF, IHC, IHC-P, IP, In vitro, KD
NB100-104
Species: Hu, Mu, Rt, Kg
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NB100-308
Species: Hu, Mu
Applications: WB, Func, ICC/IF, IP
NB100-56155
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-88707
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB100-148
Species: Hu, Mu, Rt, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, KD
NBP1-30032
Species: Hu, Mu, Bv, Ca, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
AF1085
Species: Mu
Applications: WB, IHC, Neut
NBP2-03417
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-56585
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-189
Species: Hu
Applications: WB, IHC-P, IP
NBP2-16391
Species: Hu, Mu
Applications: WB, ICC/IF
NB100-56888
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
NBP1-76905
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-33037
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CtIP Antibody (NBP1-41205)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, IP, WB.


Filter By Application
ChIP
(3)
IP
(3)
WB
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species

Reviews for CtIP Antibody (NBP1-41205) (0)

There are no reviews for CtIP Antibody (NBP1-41205). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for CtIP Antibody (NBP1-41205) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional CtIP Products

Array NBP1-41205

Bioinformatics Tool for CtIP Antibody (NBP1-41205)

Discover related pathways, diseases and genes to CtIP Antibody (NBP1-41205). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CtIP Antibody (NBP1-41205)

Discover more about diseases related to CtIP Antibody (NBP1-41205).
 

Pathways for CtIP Antibody (NBP1-41205)

View related products by pathway.

PTMs for CtIP Antibody (NBP1-41205)

Learn more about PTMs related to CtIP Antibody (NBP1-41205).

Blogs on CtIP

There are no specific blogs for CtIP, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CtIP Antibody (19E8) and receive a gift card or discount.

Bioinformatics

Gene Symbol RBBP8
Entrez
Novus 100% Guarantee