Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate-buffered saline, pH 7.4, containing no preservatives
Protein G purified
For WB: Use at a dilution of 1:500-1:1000 in 3% Milk/TBST overnight. Chromatin Immunoprecipitation and Immunoprecipitation were reported in scientific literature. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Read Publications using NBP1-41205 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for CtIP Antibody (19E8)
DNA endonuclease RBBP8
retinoblastoma binding protein 8
Retinoblastoma-binding protein 8
Retinoblastoma-interacting protein and myosin-like
Sporulation in the absence of SPO11 protein 2 homolog
CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disruppted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.