CTGF/CCN2 Antibody (2191A) [Unconjugated]

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, SVEC4-10 mouse vascular endothelial cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat CTGF/CCN2 Monoclonal Antibody (Catalog # MAB91901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CTGF/CCN2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 2191A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CTGF/CCN2 Antibody (2191A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human CTGF/CCN2
  Glu247-Ala349
  Accession # P29279
• Specificity: Detects human CTGF/CCN2 in direct ELISAs. Detects human, mouse, and rat CTGF/CCN2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CTGF/CCN2 Antibody (2191A) [Unconjugated]

• CCN2
• CCN2IGFBP-8
• connective tissue growth factor
• CTGF
• CTGRP
• Fisp12
• HCS24
• Hypertrophic chondrocyte-specific protein 24
• IBP-8
• IGF-binding protein 8
• IGFBP-8
• IGFBP8CCN family member 2
• Insulin-like growth factor-binding protein 8
• MGC102839
• NOV2

Background

CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTGF/CCN2 Antibody (MAB91901)(2)

Publications using MAB91901

• Yin, JB;Wang, YX;Fan, SS;Shang, WB;Zhu, YS;Peng, XR;Zou, C;Zhang, X; Geniposide ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-?/Smad and p38MAPK signaling pathways PloS one 2024-09-06 [PMID: 39240867] (Western Blot, Mouse)
• G Jacquemin, A Wurmser, M Huyghe, W Sun, Z Homayed, C Merle, M Perkins, F Qasrawi, S Richon, F Dingli, G Arras, D Loew, D Vignjevic, J Pannequin, S Fre Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme Elife, 2022-05-11;11(0):. 2022-05-11 [PMID: 35543624] (Neutralization, Mouse)

FAQs for CTGF/CCN2 Antibody (MAB91901). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
   • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

Bioinformatics

• Uniprot:
  • P29279()