CTCF Antibody

Images

 
Western Blot: CTCF Antibody [NB500-177] - Detection of Mouse CTCF by Western Blot. Samples: Whole cell lysate (50 ug) from CT26.WT, 4T1, Renca, and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody ...read more
Immunohistochemistry: CTCF Antibody [NB500-177] - Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 CTCFfs20 used at a dilution ...read more
Chromatin Immunoprecipitation: CTCF Antibody [NB500-177] - Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, ...read more
Western Blot: CTCF Antibody [NB500-177] - Samples: A) Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 lot 2) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CTCF Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 650 and 700 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTCF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Publications
Read Publication using
NB500-177 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CTCF Antibody

  • 11 zinc finger transcriptional repressor
  • 11-zinc finger protein
  • 11-Zinc Finger Protein2
  • CCCTC-binding factor (zinc finger protein)
  • CCCTC-binding Factor
  • CTCF
  • CTCFL paralog
  • transcriptional repressor CTCF

Background

The vertebrate insulator protein, CCCTC binding factor (CTCF) is a highly conserved, ubiquitously expressed DNA-binding protein that has widespread capabilities in gene regulation. CTCF contains 11 zinc finger motifs and it is thought that CTCF uses different combinations of zinc fingers to recognise a variety of specific sequences on the DNA to control several important genes. CTCF plays dual roles in cell growth regulatory processes and epigenetic functions. CTCF is a negative regulator of cell proliferation and is located in a region of the chromosome that is frequently deleted in human cancers. The CTCF protein is altered in some breast and prostate tumours. Substitutions from one amino acid to another in the DNA-binding domain change the whole spectrum of the genes regulated by CTCF which can result in cancer development. CTCF was first identified as one of the nuclear factors binding to the regulatory regions of the c-myc gene. Further study of this protein revealed roles in transcriptional repression, insulator function, and imprinting genetic information. Among the multitude of normal CTCF functions, its involvement in partitioning the genome into active or inactive domains by means of a chromatin insulator function has received particular attention. As an insulator CTCF helps to define the boundaries between the neighbouring genes. Strikingly, all vertebrate chromatin insulators identified so far interact with CTCF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CTCF Antibody (NB500-177)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, IP, WB.


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ChIP
(1)
IP
(1)
WB
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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Secondary Antibodies

 

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Diseases for CTCF Antibody (NB500-177)

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Bioinformatics

Gene Symbol CTCF
Entrez
Uniprot