| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44027 | |||
| BSA Free | NBP2-52911-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44025 | |||
| BSA Free | NBP2-52909-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44026 | |||
| BSA Free | NBP2-52910-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6369-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33854-25ul | |||
![Western Blot: CTCF Antibody (CL0307) [NBP2-52911] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Western-Blot-NBP2-52911-img0007.jpg "Western Blot: CTCF Antibody (CL0307) [NBP2-52911] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0307) [NBP2-52911] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunocytochemistry-Immunofluorescence-NBP2-52911-img0012.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Analysis of CTCF antibody on Human fallopian tube tissue. Antigen retrieval was done enzymatically. Image from verified customer review.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0013.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0008.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli and tubuli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0009.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0010.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0011.jpg)
![Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Western-Blot-NBP2-76420-img0001.jpg "Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311310.jpg)
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311923.jpg)
![Immunocytochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE MCF-7 cells by ICC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunocytochemistry-NBP2-76420-img0003.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0004.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) and rabbit anti-CTCF (NB500-177).](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunoprecipitation-NBP2-76420-img0005.jpg)
![Flow Cytometry-CTCF Antibody (BLR041F) [NBP2-76420] -Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] or isotype control (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-flow-cytometry-287202312246..jpg)
![Western Blot: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NB500-177-img0011.jpg "Western Blot: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunohistochemistry-NB500-177-img0013.jpg)
![Chromatin Immunoprecipitation: CTCF Antibody [NB500-177] - Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 20 micrograms of rabbit anti-CTCF antibody NB500-177. The resulting ChIP DNA was quantified using real-time PCR with primers against the IGF2-H19 ICR or a control region in the HoxA cluster.](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NB500-177-img0004.jpg)
![Western Blot: CTCF Antibody [NB500-177] - Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NB500-177-img0010.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunohistochemistry-NB500-177-img0012.jpg)
![Immunoprecipitation: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 (lot NB500-177-6) used for IP at 6 ug per reaction. CTCF was also immunoprecipitated by a previous lot of this antibody (lot NB500-177-5). For blotting immunoprecipitated CTCF, NB500-177 was used at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunoprecipitation-NB500-177-img0009.jpg)
![Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Western-Blot-NBP2-52909-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0304) [NBP2-52909] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunocytochemistry-Immunofluorescence-NBP2-52909-img0011.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0009.jpg)
![Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Western-Blot-NBP2-52909-img0004.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0006.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and glomeruli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0007.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0304-Immunohistochemistry-Paraffin-NBP2-52909-img0008.jpg)
![Western Blot: CTCF Antibody (CL0305) [NBP2-52910] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Western-Blot-NBP2-52910-img0007.jpg "Western Blot: CTCF Antibody (CL0305) [NBP2-52910] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0305) [NBP2-52910] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunocytochemistry-Immunofluorescence-NBP2-52910-img0012.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0011.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0008.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubuli and glomeruli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0009.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0010.jpg)
![Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Western-Blot-NBP3-15795-img0003.jpg "Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of NIH-3T3 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunocytochemistry-Immunofluorescence-NBP3-15795-img0002.jpg)
![Chromatin Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (NBP3-15795) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Chromatin-Immunoprecipitation-NBP3-15795-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of C6 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunocytochemistry-Immunofluorescence-NBP3-15795-img0001.jpg)
![Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody (NBP3-15795). Western blot was performed from the immunoprecipitate using CTCF antibody (NBP3-15795) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunoprecipitation-NBP3-15795-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![SDS-Page: Recombinant Human CTCF Protein [H00010664-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-CTCF-Protein-SDS-Page-H00010664-P01-img0002.jpg "SDS-Page: Recombinant Human CTCF Protein [H00010664-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody [NBP2-33854] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33854-img0005.jpg "Immunocytochemistry/Immunofluorescence: CTCF Antibody [NBP2-33854] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry: CTCF Antibody [NBP2-33854] - Staining of human colon shows strong nuclear and moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunohistochemistry-NBP2-33854-img0002.jpg)
![SDS-Page: Recombinant Human CTCF Protein [H00010664-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010664-Q01-1.jpg "SDS-Page: Recombinant Human CTCF Protein [H00010664-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation: CTCF Antibody [NB500-175] - Immunoprecipitation of Human CTCF. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-175 used at 3 mcg/mg lysate for IP. Human CTCF was also immunoprecipitated using affinity purified rabbit anti-CTCF antibodies NB500-177 and NB500-194 CTCF was blotted using NB500-177 at 0.03 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunoprecipitation-NB500-175-img0002.jpg "Immunoprecipitation: CTCF Antibody [NB500-175] - Immunoprecipitation of Human CTCF. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-175 used at 3 mcg/mg lysate for IP. Human CTCF was also immunoprecipitated using affinity purified rabbit anti-CTCF antibodies NB500-177 and NB500-194 CTCF was blotted using NB500-177 at 0.03 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.")