CS Citrate Synthase Antibody (1081813) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 1081813
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

CS Citrate Synthase Antibody (1081813) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects a synthetic peptide corresponding to residues near amino acid 400 of human CS protein in Direct ELISA.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CS Citrate Synthase Antibody (1081813) [Alexa Fluor™ Plus 647]

• citrate (Si)-synthase
• citrate synthase
• citrate synthase, mitochondrial
• CS Citrate Synthase
• EC 2.3.3
• EC 2.3.3.1

Background

Citrate Synthase (CS) is a 52kDa enzyme found in nearly all cells capable of oxidative metabolism. This enzyme is the first step of the tricarboxylic acid (TCA) cycle and is found in the mitochondrial matrix.  Citrate Synthase is commonly used as a marker for the presence of intact mitochondria. Loss of CS facilitates cytosolic glycolysis and results in epithelial-mesenchymal transition (EMT) and tumor malignancy.

1. Wiegand G, Remington SJ. Citrate synthase: structure, control, and mechanism. Annu Rev Biophys Biophys Chem. 1986;15:97-117. doi: 10.1146/annurev.bb.15.060186.000525. PMID: 3013232. 
2. Gillen JB, Martin BJ, MacInnis MJ, Skelly LE, Tarnopolsky MA, Gibala MJ. Twelve Weeks of Sprint Interval Training Improves Indices of Cardiometabolic Health Similar to Traditional Endurance Training despite a Five-Fold Lower Exercise Volume and Time Commitment. PLoS One. 2016 Apr 26;11(4):e0154075. doi: 10.1371/journal.pone.0154075. PMID: 27115137; PMCID: PMC4846072. 
3. Zhou L, Ou S, Liang T, Li M, Xiao P, Cheng J, Zhou J, Yuan L. MAEL facilitates metabolic reprogramming and breast cancer progression by promoting the degradation of citrate synthase and fumarate hydratase via chaperone-mediated autophagy. FEBS J. 2023 Jul;290(14):3614-3628. doi: 10.1111/febs.16768. Epub 2023 Mar 12. PMID: 36866961.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.