CRTH-2/GPR44 Antibody - BSA Free

Western Blot: GPR44 Antibody [NB100-93564] - WB detection of GRP44 in membrane fractions prepared from GRP44 / CRTH2 transiently transfected HEK 293 cells (Lane 1) and non-transfected HEK 293 cells ( Lane 2) using GPR44 polyclonal antibody NB100-93564 at a dilution of 1:1000 (overnight incubation at 4C ).

Western Blot: GPR44 Antibody [NB100-93564] - Analysis of GPR44 in GPR44 transfected Jurkat cells using GPR44 antibody at 1:500 dilution. Does not detect GPR44 in non-transfected parental Jurkat cells.

Product Discontinued

CRTH-2/GPR44 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to residues 329-348 [DDSELGGAGSSRRRRTSSTA] of the CRTH2 (GPR44) protein.
• Specificity: CRTH2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTGDR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is useful for Western Blot.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 43.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  TORC2 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRTH-2/GPR44 Antibody - BSA Free

• CD294 antigen
• CD294
• Chemoattractant receptor-homologous molecule expressed on Th2 cells
• CRTH2
• CRTH-2
• CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• DL1R
• DP2
• G protein-coupled receptor 44
• GPR44
• PGD2R
• PTGDR2
• putative G-protein coupled receptor 44

Background

GPCR GPR44 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. ESTs have been isolated from B-cell/lung/testis, brain, kidney, liver/spleen, placenta, prostate, testis and thymus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGDR2
• Entrez:
  • 11251(Human)
• Uniprot:
  • Q9Y5Y4()