CROT Antibody

Images

 
Western Blot: CROT Antibody [NBP1-31441] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE COT antibody, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: CROT Antibody [NBP1-31441] - Paraffin-embedded Ca922 xenograft, using antibody at 1:100 dilution.
Western Blot: CROT Antibody [NBP1-31441] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CROT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CROT. The exact sequence is proprietary.
Localization
Peroxisome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CROT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-31441 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CROT Antibody

  • carnitine O-octanoyltransferase
  • COTperoxisomal carnitine O-octanoyltransferase
  • EC 2.3.1
  • EC 2.3.1.137
  • peroxisomal carnitine acyltransferase
  • peroxisomal carnitine octanoyltransferase

Background

Carnitine octanoyltransferase (EC 2.3.1.137) is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria. See also CRAT (MIM 600184). Van der Leij et al. (2000) [PubMed 11001805] reviewed the function, structural features, and phylogenetics of human carnitine acyltransferase genes, including CROT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CROT Antibody (NBP1-31441)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CROT
Entrez
Uniprot