CROT Antibody - BSA Free

Western Blot: CROT Antibody [NBP1-31441] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE COT antibody, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-31441] - Paraffin-embedded Ca922 xenograft, using antibody at 1:100 dilution.

Western Blot: CROT Antibody [NBP1-31441] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CROT Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CROT Antibody - BSA Free (NBP1-31441) is a polyclonal antibody validated for use in IHC and WB. Anti-CROT Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CROT. The exact sequence is proprietary.
• Localization: Peroxisome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CROT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CROT Antibody - BSA Free

• carnitine O-octanoyltransferase
• COTperoxisomal carnitine O-octanoyltransferase
• EC 2.3.1
• EC 2.3.1.137
• peroxisomal carnitine acyltransferase
• peroxisomal carnitine octanoyltransferase

Background

Carnitine octanoyltransferase (EC 2.3.1.137) is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria. See also CRAT (MIM 600184). Van der Leij et al. (2000) [PubMed 11001805] reviewed the function, structural features, and phylogenetics of human carnitine acyltransferase genes, including CROT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CROT Antibody (NBP1-31441)(2)

Publications using NBP1-31441

• Price NL, Miguel V, Ding W et al. Genetic deficiency or pharmacological inhibition of miR-33 protects from kidney fibrosis JCI Insight 2019-10-15 [PMID: 31613798] (WB, Human, Mouse)
• Goedeke L, Vales-Lara FM, Fenstermaker M et al. A Regulatory Role for MicroRNA 33(Asterisk) in Controlling Lipid Metabolism Gene Expression. Mol Cell Biol 2013-06-01 [PMID: 23547260] (WB, Human)

Bioinformatics

• Gene Symbol: CROT
• Entrez:
  • 54677(Human)
  • 74114(Mouse)
• Uniprot:
  • Q9UKG9(Human)