CPVL Antibody

Western Blot: CPVL Antibody [NBP2-97080] - Lane A: 293 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: CPVL Antibody [NBP2-97080] - Staining of human CPVL in human lung with rabbit polyclonal antibody (1:10000).

Immunohistochemistry-Paraffin: CPVL Antibody [NBP2-97080] - Staining of human CPVL in human liver with rabbit polyclonal antibody (1:10000).

Immunoprecipitation: CPVL Antibody [NBP2-97080] - CPVL was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-CPVL rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CPVL rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa

Knockout Validated: CPVL Antibody [NBP2-97080] - Lane A: CPVL konckout 293FT Whole Cell Lysate Lane B: 293FT Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa. Observed band size:54 kDa. (We are unsure as to the identity of these extra bands.)

Product Discontinued

CPVL Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human CPVL (Accession#: AAH16838.1; Met1-Gly476)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPVL
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2500-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CPVL Antibody

• carboxypeptidase, vitellogenic-likeVitellogenic carboxypeptidase-like protein
• EC 3.4.16
• EC 3.4.16.-
• HVLP
• MGC10029
• probable serine carboxypeptidase CPVL
• VCP-like protein
• VLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPVL