CPT1B Antibody - BSA Free

Western Blot: CPT1B Antibody - BSA Free [NBP2-92666] - Western blot analysis of lysates from Rat skeletal muscle, using CPT1B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.

Immunohistochemistry-Paraffin: CPT1B Antibody [NBP2-92666] - Paraffin-embedded mouse heart using CPT1B .

Immunohistochemistry-Paraffin: CPT1B Antibody [NBP2-92666] - Paraffin-embedded human colon using CPT1B .

Immunocytochemistry/ Immunofluorescence: CPT1B Antibody - BSA Free [NBP2-92666] - Immunofluorescence analysis of Mouse heart tissue using CPT1B Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Immunocytochemistry/ Immunofluorescence: CPT1B Antibody - BSA Free [NBP2-92666] - Immunofluorescence analysis of Human heart tissue using CPT1B Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CPT1B Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1). SYQVAKALADDVELYCFQFLPFGKGLIKKCRTSPDAFVQIALQLAHFRDRGKFCLTYEASMTRMFREGRTETVRSCTSESTAFVQAMMEGSHTKADLRDLFQKAAKKHQNMYRLAMTGAGIDRHLFCLYLVSKYLGVSSPFLAEVLSEPWRLSTSQIPQSQIRMFDPEQHPNHLGAGGGFGPVADDGYGVSYMIAGENTIFFHISSKFSSSETNAQRFGNHIRKALLDIADLFQVPKAYS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPT1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CPT1B Antibody - BSA Free

• carnitine O-palmitoyltransferase 1, muscle isoform
• carnitine O-palmitoyltransferase I, mitochondrial muscle isoform
• Carnitine O-palmitoyltransferase I, muscle isoform
• carnitine palmitoyltransferase 1B (muscle)
• Carnitine palmitoyltransferase 1B
• Carnitine palmitoyltransferase I-like protein
• CPT I
• CPT1B
• CPT1M
• CPT1-M
• CPT1-MMCCPT1
• CPTI
• CPTI-M
• EC 2.3.1
• EC 2.3.1.21
• FLJ55729
• FLJ58750
• KIAA1670
• MCPT1
• M-CPT1

Background

The protein encoded by the CPT1B gene, a member of the carnitine/choline acetyltransferase family, is the rate-controllingenzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the nettransport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variantsencoding different isoforms have been found for this gene, and read-through transcripts are expressed from theupstream locus that include exons from this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CPT1B Antibody (NBP2-92666)(2)

Publications using NBP2-92666

• Persad KL Contribution of Energy Metabolism to Cardiomyocyte Maturation Thesis 2023-01-01 (WB, Rat)
• Berna Güven, Qiuyu Sun, Cory S Wagg, Amanda Almeida de Oliveira, Heidi Silver, Kaya L Persad, Arzu Onay-Besikci, Jennie Vu, Gavin Y Oudit, Gary D Lopaschuk Obesity Is a Major Determinant of Impaired Cardiac Energy Metabolism in Heart Failure with Preserved Ejection Fraction. The Journal of pharmacology and experimental therapeutics 2024-01-04 [PMID: 37977817]

Bioinformatics

• Gene Symbol: CPT1B